Disease related

anti-CD86 mouse monoclonal, EBS-CD-042, purified

Cat. No: 691607

Available, delivery time 1-7 days

$425.00*

Key Features
  • Mouse monoclonal
  • Suitable for FACS, ICC/IF and IHC
  • Reacts with human, mouse and rat
  • Isotype: IgG1 kappa
Product description
Quantity 1 ml (100 µg/ml)
Antibody Type Monoclonal
Host Mouse
Isotype IgG1 kappa
Conjugate Unconjugated
Application FACS, ICC/IF, IHC
Purification Affinity chromatography
Reactivity Human, Mouse, Rat
Storage 2-8°C
Intended use Research use only
Clone EBS-CD-042
Immunogen ARH-77 cells (B-lymphoblastoid cell line)
Concentration 100 µg/ml
Formulation PBS with 0.02% sodium azide
UniprotID P42081 (Human), P42082 (Mouse), O35531 (Rat)
Synonym T-lymphocyte activation antigen CD86, Activation B7-2 antigen, B70, BU63, CTLA-4 counter-receptor B7.2, FUN-1, CD antigen CD86, CD86, CD28LG2
Applications
Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) 1:100-1:200 (0.5-1.0 µg/ml)
Immunohistochemistry (IHC) - frozen 1:50-1:100 (1-2 µg/ml)
Flow Cytometry (FACS) 0.5-1.0 µg/million cells in 0.1 ml
Background

EBS-CD-42 reacts with CD86, a member of the immunoglobulin superfamily, and highly expressed on monocytes, dendritic cells and stimulated B-cells. It is probably the major CD28 ligand. Furthermore, EBS-CD-042 blocks binding of soluble CD152 to CD86.

Positive control: 293T or Jurkat cells. Monocytes and dendritic cells, activated T, B and natural killer cells in lymph node or tonsil.

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