anti-CDw78 / MHC II DR mouse monoclonal, IPO-10, purified

Cat. No: 691700

Available, delivery time 1-7 days

$425.00*

Key Features
  • Mouse monoclonal
  • Suitable for ELISA, FACS, ICC/IF and IHC
  • Reacts with human
  • Isotype: IgG3 kappa
Product description
Quantity 1 ml (100 µg/ml)
Antibody Type Monoclonal
Host Mouse
Isotype IgG3 kappa
Conjugate Unconjugated
Application ELISA, FACS, ICC/IF, IHC
Purification Affinity chromatography
Reactivity Human
Storage 2-8°C
Intended use Research use only
Clone IPO-10
Immunogen Human B-cell line Daudi
Concentration 100 µg/ml
Formulation PBS with 0.02% sodium azide
Applications
Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) 1:100-1:200 (0.5-1.0 µg/ml)
Immunohistochemistry (IHC) - frozen 1:50-1:100 (1-2 µg/ml)
Immunohistochemistry (IHC) - paraffin 1:50-1:100 (1-2 µg/ml; microwave treatment in 10 mM citrate buffer pH 6.0 recommended)
ELISA Assay dependent
Flow Cytometry (FACS) 0.5-1.0 µg/million cells in 0.1 ml
Background

CDw78 (also called Ba antigen, Leu21 or LO panB a) is present on some immature and some mature B-cells. The antigen appears on B-cell progenitors preceding CD10, CD19, CD22 and CD37. It is expressed on resting B-cells and reappears and persists in the cytoplasm and on the cell surface until cytoplasmic Ig appears. Its expression is greatly increased after B-cell activation in vitro. It is also found on tissue macrophages and on epithelial cells, but not on T-cells, NK-cells, monocytes, granulocytes, thymocytes or bone marrow stromal fibroblasts nor myeloid tissues.

Positive control: Daudi cells, Raji, Namalva, EB-3, RPMI-8226 (50% of cells), tonsil or lymph node.

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