- Mouse monoclonal
- Suitable for IHC on frozen sections
- Reacts with human
- Isotype: IgG1
Product description
| Quantity | 1 ml |
|---|---|
| Antibody Type | Monoclonal |
| Host | Mouse |
| Isotype | IgG1 |
| Conjugate | Unconjugated |
| Application | IHC |
| Purification | Hybridoma cell culture supernatant |
| Reactivity | Human |
| Storage | Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles |
| Intended use | Research use only |
| Clone | 2B6 |
| Immunogen | GST fusion protein of part of the extracellular domain of cadherin 6 (K-Cadherin) |
| Formulation | Contains 0.09% sodium azide |
| UniprotID | P55285 (Human) |
| Synonym | Cadherin-6, Kidney cadherin, K-cadherin, CDH6 |
| Note | Centrifuge prior to opening |
Applications
| Tested applications | Tested dilutions |
|---|---|
| Immunohistochemistry (IHC) - frozen | Ready-to-use (paraformaldehyde fixed; with 0.2% Triton X-100 in PBS) |
Background
The cadherin gene family encodes calcium-dependent adhesion molecules that promote homophilic interactions among cells. All cadherins are single-pass transmembrane proteins with a variable number of extracellular domains (EC; 110 amino acids long). Classical cadherins consist of five EC domains and a conserved cytoplasmic domain and are divided in type I and type II subgroups. Type I comprises cadherin E, N, P, and R. Type II include cadherin-5, -6, -8, -11, and -12.
During embryogenesis differential expression of cadherins can drive morphogenesis by stimulating cell aggregation, defining boundaries between groups of cells and promoting cell migration. Cadherin-6 seems to be required for the early aggregation of induced mesenchymal cells and their subsequent conversion to epithelium.
Positive control: Embryonic kidney (e.g. establishment of developmental pattern of nephrons)
References/Publications (2)
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FAQs
The concentration of purified antibodies is mentioned on the datasheet.
For prediluted antibodies the concentration may vary from lot to lot. The concentration of these antibodies is not mentioned on the datasheet and can be requested at support@progen.com.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.