Disease related
Highly published

anti-Cardiac Actin mouse monoclonal, AC1-20.4.2, liquid, purified, sample

Liquid

Lyophilized

Liquid

Cat. No: 690075S

Available, delivery time 1-7 days

$96.00*

Key Features
  • Mouse monoclonal
  • Suitable for IHC and WB
  • Reacts with bovine, chicken, human, mouse and rabbit
  • Isotype: IgG1
Product description
Quantity 200 µl
Antibody Type Monoclonal
Host Mouse
Isotype IgG1
Conjugate Unconjugated
Application IHC, WB
Purification Affinity chromatography
Reactivity Bovine, Chicken, Human, Mouse, Rabbit
Storage Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use Research use only
Clone AC1-20.4.2
Immunogen Synthetic NH2 terminus decapeptide of cardiac isoform of actin
Concentration 50 µg/ml (10 µg)

Formulation PBS pH 7.4 with 0.09% sodium azide and 0.5% BSA
UniprotID P68034 (Chicken), P68032 (Human), G1STB6 (Rabbit)
Synonym Actin, alpha cardiac muscle 1, Alpha-cardiac actin [Cleaved into: Actin, alpha cardiac muscle 1, intermediate form], ACTC1, ACTC
Applications
Tested applications Tested dilutions
Immunohistochemistry (IHC) - frozen 1:10 (5 µg/ml; include 0.5 M NaCl in all washing buffers to enhance specificity)
Immunohistochemistry (IHC) - paraffin 1:10 (5 µg/ml; microwave treatment recommended)
Western Blot (WB) 1:1,000 (50 ng/ml; include 1 M NaCl in all washing buffers to enhance specificity)
Background

Ac1 represents an excellent marker for cardiac tissue; it discriminates fetal (cardiac) a-actin from all other actin isoforms. Fetal actin can be localized in regenerating skeletal muscle after injury (in satellite cells) and in veins of the umbelical cord. Mab Ac1-20.4.2 shows no cross reaction with other actin isoforms present in skeletal and smooth muscle, provided that stringent experimental conditions have been applied.
Polypeptide reacting: Specific for fetal (cardiac) isoform of actin.

References/Publications (9)
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FAQs
The concentration of unpurified supernatant, ascites, unpurified guinea pig serum and unpurified rabbit serum is not determined.
The concentration of purified antibodies is mentioned on the datasheet.
For prediluted antibodies the concentration may vary from lot to lot. The concentration of these antibodies is not mentioned on the datasheet and can be requested at support@progen.com.

Most of our purified mouse antibodies contain 0.5% BSA as stabilizer. If BSA was added to the antibody solution, it is stated in the datasheet.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.

Please find the solvent for reconstitution and the amount of liquid for reconstitution on the antibody datasheet. The buffer formulation after reconstitution is also mentioned in the datasheet.
PROGEN offers different formats for mouse monoclonal antibodies.  Different antibodies may not be offered in each format, the following are all possible formats:
  1. Supernatant and supernatant concentrate: This format contains hybridoma cell culture supernatant. The antibody is not purified and the antibody concentration is not determined. The antibody concentration may vary from lot to lot. Therefore we recommend to titrate the optimal concentration for the application used for each new lot.
  2. Lyophilized, purified: This format contains purified antibody in lyophilized form. The reconstitution of this antibody is described in the datasheet. The buffer composition after reconstitution is also mentioned on the datasheet. 
  3. Liquid, purified: This format contains purified antibody in liquid format. The concentration is mentioned on the datasheet. 
  4. Prediluted, purified: This format contains purified antibody in liquid format. Most antibodies in this format are diluted to be ready-to-use for IHC with standard tissue. But some antibodies of this format need further dilution for IHC. This is mentioned on the datasheet.

Lyophilized antibodies can be stored at 2-8°C until expiration.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.

The expiration date of our antibodies is indicated on the product label.
We offer bulk amounts starting from 1 mg for many of our mouse monoclonal or recombinant antibodies. Please contact us for further information or use the “bulk button” on the product page?
Most of our antibodies contain 0.09% sodium azide as preservative. If a preservative is added, it is mentioned in the datasheet.
  • The addition of 1 M NaCl during the first antibody incubation step and the subsequent washing steps in order to eliminate binding to sarcomeric actin is recommended. The epitope of antibody Ac1-20.4.2 on cardiac actin (located at the very end of the N-terminus) differs only by 1 amino acid from the sarcomeric actin.
  • To increase stringency further washing steps could be added.
  •  It is recommended to omit Tween during the antibody incubation step. In our routine protocols we use PBS, supplemented with 1 M NaCl for primary antibody incubation (1 hour) and 3 washing steps (5 min) with PBS/ 1M NaCl.

The optimal antibody dilution for your specific protocol and application needs to be titrated in your lab with your equipment and sample. The optimal dilution may vary between protocols and samples. A good dilution for starting the titration is the dilution mentioned in the datasheet. If the sample needs a specific treatment (eg. Antigen retrieval for IHC on FFPE sections) this should also be mentioned on the antibody datasheet. 
PROGEN antibodies are shipped at ambient temperature. The antibodies are stable at ambient temperature for the shipment period. Please store the antibodies as indicated in the datasheet upon arrival.
Cardiac Actin, bovine, 100 µg
Cat. No: 62224
  • Purified bovine cardiac actin
  • Protein standard

$293.00*