Epitope mapped
Disease related

anti-Chlamydia Multiepitope Cocktail mouse monoclonal, ACI, ICK, 502, FITC Conjugate

Cat. No: FR97457

Available, delivery time 1-7 days

$169.00*

Key Features
  • Mouse monoclonal
  • Suitable for ICC/IF and IHC
  • Reacts with Chlamydia genus
  • Isotype: IgG1 and IgG3
Product description
Quantity 1.5 ml
Antibody Type Monoclonal
Host Mouse
Isotype IgG1 and IgG3
Conjugate FITC
Application ICC/IF, IHC
Purification Affinity chromatography
Reactivity Chlamydia genus
Storage 2-8°C
Intended use Research use only
Clone ACI, ICK, 502
Immunogen Chlamydia antigen
Formulation Lyophilized; reconstitute in 1.5 ml dist. water (final solution contains 0.09% sodium azide, 0.5% BSA in PBS buffer, pH 7.4); contains Evans blue as counterstain
Applications
Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) Ready-to-use
Immunohistochemistry (IHC) - frozen Ready-to-use
Immunohistochemistry (IHC) - paraffin Ready-to-use
Background

The multi-epitope cocktail detects the elementary bodies (Ebs), reticulate bodies (RBs), intermediate forms, chlamydial inclusions and specific cell-associated anti-gen(s) directly in samples. The conjugate is used for direct immunofluorescence staining combining three specific monoclonal antibodies (mab) conjugated to fluo-rescein isothiocyanate (FITC).
One mab is specific for the genus-specific epitope located on the Chlamydia LPS and identifies all the 15 known serovars of C. trachomatis as well as C. psittaci and C. pneumoniae by displaying bright fluores-cence of intracellular inclusions and pin-point shaped extracellular organisms as well as free cell-associated chlamydial antigen(s).
A second mab reacts with an epitope on the Chlamydia-outer membrane complex protein (60 kDa) and shows preferential fluorescence around the periphery of individual RBs of all serotypes of C. trachomatis and of C. psittaci strains; this antibody also stains the EBs of C-complex serovars as pin-point shaped structures.
The third mab identifies a species-specific epitope on the major outer membrane protein (40 kDa) and shows brilliant fluorescence with EBs, RBs and cytoplasmic inclusions of B-complex serovars of C. trachomatis, whereas C-complex serovars show medium to weak fluorescence.
The combination of these 3 specific mabs provides uniform and intense staining of all the stages in the developmental cycle of the known C. trachomatis serovars (A-C, D-K, L1-L3) and C. psittaci and C. pneumoniae strains. A positive cross-reactivity was also reported for Chlamydophila pecorum.

Contains Evans blue as counterstain.

References/Publications (1)
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anti-Chlamydia mouse monoclonal, 502, lyophilized, purified
Cat. No: 502-P
  • Mouse monoclonal
  • Suitable for ICC/IF and IHC
  • Reacts with C. psittaci, C. trachomatis
  • Isotype: IgG1

$153.00*
anti-Chlamydia mouse monoclonal, ICK, lyophilized, purified
Cat. No: ICK-P
  • Mouse monoclonal
  • Suitable for ICC/IF and IHC
  • Reacts with C. psittaci and C. trachomatis
  • Isotype: IgG1

$153.00*
anti-Chlamydia mouse monoclonal, ACI, lyophilized, purified, 500 µg
Cat. No: ACI-P500
Quantity: 500 µg
  • Purified, lyophilized
  • Mouse monoclonal
  • Suitable for ELISA, ICC/IF and IHC
  • Reacts with Chlamydia genus
  • Isotype: IgG3

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