Key Features
- Purified, lyophilized
- Mouse monoclonal
- Suitable for ELISA, ICC/IF and IHC
- Reacts with Chlamydia genus
- Isotype: IgG3
Product description
Quantity | 50 µg |
---|---|
Antibody Type | Monoclonal |
Host | Mouse |
Isotype | IgG3 |
Conjugate | Unconjugated |
Application | ELISA, ICC/IF, IHC |
Purification | Affinity chromatography |
Reactivity | Chlamydia genus |
Storage before reconstitution | 2-8°C until indicated expiry date |
Storage after reconstitution | 2-8°C |
Intended use | Research use only |
Clone | ACI |
Immunogen | Chlamydia antigen |
Concentration | 50 µg/ml after reconstitution with 1 ml dist. water |
Formulation | Lyophilized; reconstitute in 1 ml dist. water (final solution contains 0.09% sodium azide, 0.5% BSA in PBS buffer, pH 7.4) |
Applications
Tested applications | Tested dilutions |
---|---|
Immunocytochemistry (ICC)/ Immunofluorescence (IF) | 1:10 |
Immunohistochemistry (IHC) - paraffin | Assay dependent |
ELISA | Assay dependent |
Background
Mab ACI recognizes a genus-specific epitope of the Chlamydia lipopolysaccharide antigen and identifies all serovars (A-C, D-K, L1-L3) of C. trachomatis as well as Chlamydophila psittaci and Chlamydophila pneumoniae with a strong fluorescence of the intracellular inclusions, the pinhead-sized extracellular elementary bodies and the free cell-associated Chlamydia lipopolysaccharide antigens (amorphous foci). A positive cross-reactivity was al-so reported for Chlamydophila pecorum.
References/Publications (15)
Publication
Species
Application
Species
C. psittaci
Application
IHC (paraffin)
Species
C. spec
Application
IHC (paraffin)
Publication
Borel, N. et al. Chlamydiae in human intestinal biopsy samples. Pathog.Dis. 76,- (2018).
Species
C. trachomatis
Application
IHC (paraffin)
Species
C. suis
Application
ICC-IF
Species
C. pneumoniae
Application
ICC-IF
Species
C. abortus
Application
IHC (paraffin)
Species
C. psittaci
Application
IHC (paraffin)
Species
C. pecorum
Application
ICC-IF
Species
C. trachomatis,C. pecorum
Application
ICC-IF
Species
C. trachomatis,C. pecorum
Application
ICC-IF
Species
C. pecorum
Application
ICC-IF
Species
C. abortus,C. pecorum
Application
ICC-IF
Species
C. pneumoniae
Application
IHC (paraffin)
Species
C. pneumoniae
Application
ICC-IF
Species
C. spec
Application
IHC (paraffin)
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FAQs
Most of our purified mouse antibodies contain 0.5% BSA as stabilizer. If BSA was added to the antibody solution, it is stated in the datasheet.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.
Lyophilized antibodies can be stored at 2-8°C until expiration.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.
The expiration date of our antibodies is indicated on the product label.
Most of our antibodies contain 0.09% sodium azide as preservative. If a preservative is added, it is mentioned in the datasheet.
The optimal antibody dilution for your specific protocol and application needs to be titrated in your lab with your equipment and sample. The optimal dilution may vary between protocols and samples. A good dilution for starting the titration is the dilution mentioned in the datasheet. If the sample needs a specific treatment (eg. Antigen retrieval for IHC on FFPE sections) this should also be mentioned on the antibody datasheet.
PROGEN antibodies are shipped at ambient temperature. The antibodies are stable at ambient temperature for the shipment period. Please store the antibodies as indicated in the datasheet upon arrival.