Diagnostic marker

anti-Clathrin mouse monoclonal, CHC 5.9, lyophilized, purified

Cat. No: 61017

Available, delivery time 1-7 days


Key Features
  • Mouse monoclonal
  • Suitable for IHC and WB
  • Reacts with amphibia, bovine, human, pig and rat
  • Isotype: IgM
Product description
Quantity 100 µg
Antibody Type Monoclonal
Host Mouse
Isotype IgM
Conjugate Unconjugated
Application IHC, WB
Purification Size exclusion chromatography
Reactivity Amphibia, Bovine, Human, Pig, Rat
Storage before reconstitution 2-8°C until indicated expiry date
Storage after reconstitution Up to 3 months at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use Research use only
Clone CHC5.9
Immunogen Coated vesicles (clathrin) of bovine brain
Concentration 100 µg/ml after reconstitution with 1 ml dist. water

Formulation Lyophilized; reconstitute in 1 ml dist. water (final solution contains 0.09% sodium azide, 0.5% BSA in PBS buffer, pH 7.4)
UniprotID P49951 (Bovine), Q00610 (Human), A0A5G2R4Y7 (Pig), P11442 (Rat)
Synonym Clathrin heavy chain 1, Clathrin heavy chain on chromosome 17, CLH-17, CLTC, CLH17, CLTCL2, KIAA0034
Tested applications Tested dilutions
Immunohistochemistry (IHC) - frozen 1:10
Western Blot (WB) 1:100-1:500

CHC5.9 represents an excellent marker for detection of receptor mediated endocytosis.
Reactive polypeptide: Mr 180 kDa polypeptide (clathrin heavy chain) from coated vesicles of various tissues.
Structures and tissues specifically detected: Coated vesicles of different organs and tissues (eg brain, mammary gland, ovaries).
Reactivity on cultured cell lines: HeLa, SV-40, RVF-SMC

References/Publications (1)
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The concentration of unpurified supernatant, ascites, unpurified guinea pig serum and unpurified rabbit serum is not determined.
The concentration of purified antibodies is mentioned on the datasheet.
For prediluted antibodies the concentration may vary from lot to lot. The concentration of these antibodies is not mentioned on the datasheet and can be requested at support@progen.com.

Most of our purified mouse antibodies contain 0.5% BSA as stabilizer. If BSA was added to the antibody solution, it is stated in the datasheet.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.

Please find the solvent for reconstitution and the amount of liquid for reconstitution on the antibody datasheet. The buffer formulation after reconstitution is also mentioned in the datasheet.
  • For Cells: wash with PBS + 2 mM MgCl2 and 1.5 mM CaCl2 for stabilization of the nucleus.
  • Fix tissue sections/cells for 5 min in precooled (-20°C) methanol. Fix for 2-5 min in precooled (-20°C) aceton.
  • Air dry slide.
  • Incubate for 1 h in humid chamber with primary at RT (or overnight at 2-8°C).
  • Wash 3x with PBS.
  • Incubate for 30 min in humid chamber with appropriate dilution of secondary antibody (labelled e.g. with FITC, Cy2 or Cy3).
  • Wash 3x with PBS.
  • Air dry slide.
  • Apply mounting medium and cover with cover slip.

PROGEN offers different formats for mouse monoclonal antibodies.  Different antibodies may not be offered in each format, the following are all possible formats:
  1. Supernatant and supernatant concentrate: This format contains hybridoma cell culture supernatant. The antibody is not purified and the antibody concentration is not determined. The antibody concentration may vary from lot to lot. Therefore we recommend to titrate the optimal concentration for the application used for each new lot.
  2. Lyophilized, purified: This format contains purified antibody in lyophilized form. The reconstitution of this antibody is described in the datasheet. The buffer composition after reconstitution is also mentioned on the datasheet. 
  3. Liquid, purified: This format contains purified antibody in liquid format. The concentration is mentioned on the datasheet. 
  4. Prediluted, purified: This format contains purified antibody in liquid format. Most antibodies in this format are diluted to be ready-to-use for IHC with standard tissue. But some antibodies of this format need further dilution for IHC. This is mentioned on the datasheet.

Lyophilized antibodies can be stored at 2-8°C until expiration.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.

The expiration date of our antibodies is indicated on the product label.
We offer bulk amounts starting from 1 mg for many of our mouse monoclonal or recombinant antibodies. Please contact us for further information or use the “bulk button” on the product page?
Most of our antibodies contain 0.09% sodium azide as preservative. If a preservative is added, it is mentioned in the datasheet.
The optimal antibody dilution for your specific protocol and application needs to be titrated in your lab with your equipment and sample. The optimal dilution may vary between protocols and samples. A good dilution for starting the titration is the dilution mentioned in the datasheet. If the sample needs a specific treatment (eg. Antigen retrieval for IHC on FFPE sections) this should also be mentioned on the antibody datasheet. 
PROGEN antibodies are shipped at ambient temperature. The antibodies are stable at ambient temperature for the shipment period. Please store the antibodies as indicated in the datasheet upon arrival.