Frequently used

anti-Collagen Type IV rabbit polyclonal, serum

Cat. No: 10760

Available, delivery time 1-7 days


Key Features
  • Rabbit polyclonal
  • Suitable for IHC
  • Reacts with humans, mouse and rat
Product description
Quantity 250 µl
Antibody Type Polyclonal
Host Rabbit
Conjugate Unconjugated
Application IHC
Purification Stabilized antiserum
Reactivity Human, Mouse, Rat
Storage Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use Research use only
Immunogen Isolated from human placenta
Formulation Contains 0.09% sodium azide
UniprotID P02462 (Human),P02463 (Mouse),F1MA59 (Rat)
Synonym Collagen alpha-1(IV chain [Cleaved into: Arresten], COL4A1
Note Centrifuge prior to opening
Tested applications Tested dilutions
Immunohistochemistry (IHC) - frozen 1:50 - 1:100
Immunohistochemistry (IHC) - paraffin 1:50 - 1:100 (proteolytic treatment required)

The antibody is reactive with collagen type IV of basement membranes, and shows a homogeneous staining pattern in all tissues. As neoplastic cells of invasive carcinomas often lack a continuous basement membrane, the antiserum is useful to distinguish between non-invasive and invasive lesions. Additionally, it can be used for the differentiation of bullous lesions in dermatopathology. In immunohistochemistry no cross-reactivity with other collagens at optimal dilutions. In immunoblotting, a slight cross-reactivity with collagen type V is observed.

Positive control: Skin, kidney.

References/Publications (8)
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anti-rabbit IgG goat polyclonal, HRP conjugate
Cat. No: 90003
  • HRP conjugate
  • Goat polyclonal
  • Suitable for ELISA and WB
  • Reacts with rabbit

anti-Collagen Type IV mouse monoclonal, 1042.0, supernatant
Cat. No: 10710
  • Mouse monoclonal
  • Suitable for IHC
  • Reacts with human
  • Isotype: IgG2b