anti-Complement C1q mouse monoclonal, 242G3, lyophilized, purified

anti-Complement C1q mouse monoclonal, 242G3, lyophilized, purified View more

Cat. No: 610136

Prices excl. tax

Available, delivery time 1-7 days

$245.00*

Datasheet

Key Features

Mouse monoclonal
Suitable for ELISA and WB
Reacts with human
Isotype: IgG1

Product description

Quantity	50 µg
Antibody Type	Monoclonal
Host	Mouse
Isotype	IgG1
Conjugate	Unconjugated
Application	ELISA, WB
Purification	Affinity chromatography
Reactivity	Human
Storage before reconstitution	2-8°C until indicated expiry date
Storage after reconstitution	Up to 3 months at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use	Research use only
Clone	242G3
Immunogen	Purified human complement C1q
Concentration	50 µg/ml after reconstitution with 1 ml dist. water
Formulation	Lyophilized; reconstitute in 1 ml dist. water (final solution contains 0.09% sodium azide, 0.5% BSA in PBS buffer, pH 7.4)
UniprotID	O75973 (Human)
Synonym	C1q-related factor, C1q and tumor necrosis factor-related protein 14, C1q/TNF-related protein 14, Complement component 1 Q subcomponent-like 1, C1QL1, C1QRF, CRF, CTRP14

Applications

Tested applications	Tested dilutions
ELISA	Assay dependent
Western Blot (WB)	1:100

Background

Detection of C1q in serum, in serum-C1 and within immune complexes; inhibits hemolytic activity of C1 by binding to C1q in the liquid phase (antibody concentration necessary to inhibit 50% of hemolytic activity in the fluid phase is 0.85 µg/ml). With C1 bound to immune complexes a dose dependent increase of C1 activity was observed at antibody concentrations of 1.5 µg/ml. Useful for detection of C1q-bearing immune complexes in patients with rheumatic disorders.

Polypeptide reacting: C-chain of complement C1q (25 kD in SDS-PAGE)

References/Publications (3)

Publication

Species

Application

Publication Antes, U., Heinz, H. P. & Loos, M. Detection of C1q-bearing immune complexes by a monoclonal anti-C1q ELISA system. J. Immunol. Methods 102, 149–156 (1987).

Species human

Application ELISA

Publication Heinz, H. P., Burger, R., Golan, M. D. & Loos, M. Activation of the first component of complement, C1, by a monoclonal antibody recognizing the C chain of C1q. J. Immunol. 132, 804–8 (1984).

Species human

Application WB

Publication Antes, U., Heinz, H. P. & Loos, M. Enzyme-linked immunosorbent assay for C1q in human serum by use of monoclonal antibodies. J. Immunol. Methods 74, 299–306 (1984).

Species human

Application ELISA

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Datasheet

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FAQs

Dry milk is not recommended since normal bovine milk contains approximately 250 ng/ml C1q, which would also react with the antibody. Please test other blocking solutions.

The concentration of unpurified supernatant, ascites, unpurified guinea pig serum and unpurified rabbit serum is not determined.
The concentration of purified antibodies is mentioned on the datasheet.
For prediluted antibodies the concentration may vary from lot to lot. The concentration of these antibodies is not mentioned on the datasheet and can be requested at support@progen.com.

Most of our purified mouse antibodies contain 0.5% BSA as stabilizer. If BSA was added to the antibody solution, it is stated in the datasheet.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.

Please find the solvent for reconstitution and the amount of liquid for reconstitution on the antibody datasheet. The buffer formulation after reconstitution is also mentioned in the datasheet.

PROGEN offers different formats for mouse monoclonal antibodies. Different antibodies may not be offered in each format, the following are all possible formats:

Supernatant and supernatant concentrate: This format contains hybridoma cell culture supernatant. The antibody is not purified and the antibody concentration is not determined. The antibody concentration may vary from lot to lot. Therefore we recommend to titrate the optimal concentration for the application used for each new lot.
Lyophilized, purified: This format contains purified antibody in lyophilized form. The reconstitution of this antibody is described in the datasheet. The buffer composition after reconstitution is also mentioned on the datasheet.
Liquid, purified: This format contains purified antibody in liquid format. The concentration is mentioned on the datasheet.
Prediluted, purified: This format contains purified antibody in liquid format. Most antibodies in this format are diluted to be ready-to-use for IHC with standard tissue. But some antibodies of this format need further dilution for IHC. This is mentioned on the datasheet.

Lyophilized antibodies can be stored at 2-8°C until expiration.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.

The expiration date of our antibodies is indicated on the product label.

We offer bulk amounts starting from 1 mg for many of our mouse monoclonal or recombinant antibodies. Please contact us for further information or use the “bulk button” on the product page.

Most of our antibodies contain 0.09% sodium azide as preservative. If a preservative is added, it is mentioned in the datasheet.

The optimal antibody dilution for your specific protocol and application needs to be titrated in your lab with your equipment and sample. The optimal dilution may vary between protocols and samples. A good dilution for starting the titration is the dilution mentioned in the datasheet. If the sample needs a specific treatment (eg. Antigen retrieval for IHC on FFPE sections) this should also be mentioned on the antibody datasheet.

PROGEN antibodies are shipped at ambient temperature. The antibodies are stable at ambient temperature for the shipment period. Please store the antibodies as indicated in the datasheet upon arrival.

Progen Support

For scientific and customer support, you can reach us by phone or email.

anti-Complement C1q mouse monoclonal, 242G3, lyophilized, purified

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