anti-Cylicin I mouse monoclonal, Cal-144, supernatant

Cat. No: 65142

Available, delivery time 1-7 days

$174.00*

Key Features
  • Mouse monoclonal
  • Suitable for ICC/IF and WB
  • Reacts with bovine and human
  • Isotype: IgM
Product description
Quantity 5 ml
Antibody Type Monoclonal
Host Mouse
Isotype IgM
Conjugate Unconjugated
Application ICC/IF, WB
Purification Hybridoma cell culture supernatant
Reactivity Bovine, Human
Storage Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use Research use only
Clone Cal-144 (X 144.3.2)
Immunogen Calyx preparation from bovine sperm (according to Longo et al. 1987, JCB 22, 1105-1120)
Formulation Contains 0.09% sodium azide
Applications
Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) Ready-to-use
Western Blot (WB) Assay dependent
Background

Especially useful in discrimination of morphologically altered "round-headed" spermatozoa.

Polypeptides reacting: bovine cylicin I (Mr 74,788, deduced from sequence analysis), a basic protein (IEP 10.55) of  the sperm head cytoskeleton with Mr 58,000 on SDS-PAGE; multiple band proteins with MW 58, 63, 69 and 74 kDa of calyx-associated polypeptides of bovine spermatozoa.

References/Publications (1)
Publication
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