Highly published

anti-DNA mouse monoclonal, AC-30-10, liquid, purified

Liquid

Lyophilized

Liquid

Cat. No: 690014

Available, delivery time 1-7 days

$304.00*

Key Features
  • Mouse monoclonal
  • Suitable for Dot Blots, ICC/IF and IHC
  • Reacts with all species
  • Isotype: IgM
Product description
Quantity 1 ml
Antibody Type Monoclonal
Host Mouse
Isotype IgM
Conjugate Unconjugated
Application Dot blot, ICC/IF, IHC
Purification Size exclusion chromatography
Reactivity All species
Storage 2-8°C
Intended use Research use only
Clone AC-30-10
Immunogen Double- and single-stranded DNA
Concentration 100 µg/ml (100 µg)
Formulation PBS pH 7.4 with 0.09% sodium azide and 0.5% BSA
Applications
Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) 1:200 (0.5 µg/ml)
Immunohistochemistry (IHC) - frozen 1:10 (10 µg/ml)
Immunohistochemistry (IHC) - paraffin 1:10 (10 µg/ml, microwave treatment recommended)
Dot Blot Assay dependent (on nitrocellulose membrane, after baking at 70°C)
Background


AC-30-10 stains DNA present in the nuclei and the mitochondria of cells and tissues. It is also a reliable positive control in assays for detection of DNA antibodies, especially for the detection of autoantibodies in autoimmune diseases. Due to the high sensitivity it can also be used for the detection of mycoplasma contamination in cell cultures.
Binding characteristics: reacts with all forms of native and denatured DNA; reactive also with lambda DNA and synthetic DNA.
Tested cultured cell lines: XLKE-A6 (Xenopus), RVF-SMC, PTK-2, HeLa.

References/Publications (23)
Publication
Species
Application
Species mouse,rat,rat,xenopus,Crithidia cuciliae,Triturus,chicken,bovine,M13 single and double stranded,recombinant plasmid DNA
Application IHC (frozen),IF,ICC-IF,IEM,dot blot
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FAQs
The concentration of unpurified supernatant, ascites, unpurified guinea pig serum and unpurified rabbit serum is not determined.
The concentration of purified antibodies is mentioned on the datasheet.
For prediluted antibodies the concentration may vary from lot to lot. The concentration of these antibodies is not mentioned on the datasheet and can be requested at support@progen.com.

Most of our purified mouse antibodies contain 0.5% BSA as stabilizer. If BSA was added to the antibody solution, it is stated in the datasheet.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.

Please find the solvent for reconstitution and the amount of liquid for reconstitution on the antibody datasheet. The buffer formulation after reconstitution is also mentioned in the datasheet.
PROGEN offers different formats for mouse monoclonal antibodies.  Different antibodies may not be offered in each format, the following are all possible formats:
  1. Supernatant and supernatant concentrate: This format contains hybridoma cell culture supernatant. The antibody is not purified and the antibody concentration is not determined. The antibody concentration may vary from lot to lot. Therefore we recommend to titrate the optimal concentration for the application used for each new lot.
  2. Lyophilized, purified: This format contains purified antibody in lyophilized form. The reconstitution of this antibody is described in the datasheet. The buffer composition after reconstitution is also mentioned on the datasheet. 
  3. Liquid, purified: This format contains purified antibody in liquid format. The concentration is mentioned on the datasheet. 
  4. Prediluted, purified: This format contains purified antibody in liquid format. Most antibodies in this format are diluted to be ready-to-use for IHC with standard tissue. But some antibodies of this format need further dilution for IHC. This is mentioned on the datasheet.

Lyophilized antibodies can be stored at 2-8°C until expiration.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.

The expiration date of our antibodies is indicated on the product label.
We offer bulk amounts starting from 1 mg for many of our mouse monoclonal or recombinant antibodies. Please contact us for further information or use the “bulk button” on the product page?
  • The antibody is very well suited for the detection of mitochondrial DNA; it is extremely sensitive and the signal of mitochondrial DNA is much stronger as mtDNA is not packed as densely as the DNA in the nucleus. The antibody stains the DNA in the nucleus weaker as the mitochondrial DNA.
  • For staining of the nucleus fixation with methanol/acetone (5 min in methanol (-20°C) and 5 min in acetone (-20°C)) is recommended.
  • The antibody is also suitable to stain bacterial DNA or synthetic single stranded DNA.

Most of our antibodies contain 0.09% sodium azide as preservative. If a preservative is added, it is mentioned in the datasheet.
The optimal antibody dilution for your specific protocol and application needs to be titrated in your lab with your equipment and sample. The optimal dilution may vary between protocols and samples. A good dilution for starting the titration is the dilution mentioned in the datasheet. If the sample needs a specific treatment (eg. Antigen retrieval for IHC on FFPE sections) this should also be mentioned on the antibody datasheet. 
PROGEN antibodies are shipped at ambient temperature. The antibodies are stable at ambient temperature for the shipment period. Please store the antibodies as indicated in the datasheet upon arrival.