Epitope mapped
Disease related
Frequently used

anti-Desmoglein 1/2 mouse monoclonal, DG 3.10, purified, sample (ready-to-use)

Lyophilized

Sample

Cat. No: 61002S

Available, delivery time 1-7 days

$65.00*

Key Features
  • Purified, prediluted sample, ready-to-use for IHC
  • Mouse monoclonal
  • Suitable for ICC/IF, IHC and WB
  • Reacts with bovine, human, rat
  • Isotype: IgG1
Product description
Quantity 600 µl (ready-to-use)
Antibody Type Monoclonal
Host Mouse
Isotype IgG1
Conjugate Unconjugated
Application ICC/IF, IHC, WB
Purification Affinity chromatography
Reactivity Bovine, Human, Rat
Storage Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use Research use only
Clone DG 3.10
Immunogen "Band 3" polypeptide of isolated desmosomes from bovine muzzle epidermis
Formulation PBS pH 7.4 with 0.5% BSA and 0.09% sodium azide
UniprotID Q03763 (Bovine),Q02413 (Human),D3ZM39 (Rat)
Synonym Desmoglein-1, Cadherin family member 4, Desmosomal glycoprotein 1, DG1, DGI, Pemphigus foliaceus antigen, DSG1, CDHF4
Applications
Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) Assay dependent
Immunohistochemistry (IHC) - frozen Ready-to-use
Immunohistochemistry (IHC) - paraffin Ready-to-use (microwave treatment recommended)
Western Blot (WB) Assay dependent
Background

DG 3.10 recognizes desmoglein 1 + 2 (desmosome-specific cadherins) in desmosome-bearing cells; does not react with cells containing plaque-bearing junctions of non-desmosomal type. Tumors specifically detected: all epithelium-derived tumors, notably carcinomas, and meningiomas. Polypeptide reacting: Mr 165 000 Desmoglein, "band 3" polypeptide of the desmosomal complex. Epitope recognized has been localized at the repeating unit domain ("RUD") of the cytoplasmic C-terminal part of the polypeptide.

Reactivity on cultured cell lines: A-431, MDBK.

References/Publications (23)
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Species
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Category Protocol
Size 924.86 KB
Filetype pdf
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