anti-Desmoglein 2 mouse monoclonal, G96, supernatant
- Mouse monoclonal
- Suitable for ELISA, IHC and WB
- Reacts with human
- Isotype: IgG1
Product description
| Quantity | 5 ml |
|---|---|
| Antibody Type | Monoclonal |
| Host | Mouse |
| Isotype | IgG1 |
| Conjugate | Unconjugated |
| Application | ELISA, IHC, WB |
| Purification | Hybridoma cell culture supernatant |
| Reactivity | Human |
| No reactivity | Mouse, Rat |
| Storage | Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles |
| Intended use | Research use only |
| Clone | G96 |
| Immunogen | Recombinant peptide (220aa of 2nd and 3rd extracellular repeat domain) |
| Formulation | Contains 0.09% sodium azide |
| UniprotID | Q14126 (Human) |
| Synonym | Desmoglein-2, Cadherin family member 5, HDGC, DSG2, CDHF5 |
Applications
| Tested applications | Tested dilutions |
|---|---|
| Immunohistochemistry (IHC) - frozen | Ready-to-use (for better resolution preincubation (directliy after fixation) with 0.05-0.2% Triton X-100, for 5-10 min, depending on tissue type, is recommended) |
| Immunohistochemistry (IHC) - paraffin | Ready-to-use (microwave treatment recommended) |
| ELISA | Assay dependent |
| Western Blot (WB) | Assay dependent |
Background
Antibody recognizes desmoglein 2 present in all epithelia and in tumors derived therefrom as well as in heart muscle and dendritic reticulum cells of lymphatic follicles. In basal epithelial cells desmoglein 2 expression is generally very low.
Polypeptide reacting: Desmoglein 2 (1069aa; calculated mol. wt. 116,760).
Recommended as positive control: CaCo-2, HaCaT.
Reactivities on Cultured cell lines: Many, including CaCo-2, HaCaT, MCF-7.
References/Publications (3)
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FAQs
The concentration of purified antibodies is mentioned on the datasheet.
For prediluted antibodies the concentration may vary from lot to lot. The concentration of these antibodies is not mentioned on the datasheet and can be requested at support@progen.com.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.
- Supernatant and supernatant concentrate: This format contains hybridoma cell culture supernatant. The antibody is not purified and the antibody concentration is not determined. The antibody concentration may vary from lot to lot. Therefore we recommend to titrate the optimal concentration for the application used for each new lot.
- Lyophilized, purified: This format contains purified antibody in lyophilized form. The reconstitution of this antibody is described in the datasheet. The buffer composition after reconstitution is also mentioned on the datasheet.
- Liquid, purified: This format contains purified antibody in liquid format. The concentration is mentioned on the datasheet.
- Prediluted, purified: This format contains purified antibody in liquid format. Most antibodies in this format are diluted to be ready-to-use for IHC with standard tissue. But some antibodies of this format need further dilution for IHC. This is mentioned on the datasheet.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.
- Homogenization of tissue in PBS (supplemented with protease inhibitors).
- Centrifugation at 10E4 x g.
- Optional: treat pellet with DNase.
- Resuspend pellet in detergent buffer (e.g. 1% Triton X-100), stir for 1 h at 2-8°C.
- Centrifugation at 10E4 x g.
- Resuspend pellet in high salt buffer (with 1.5 M KCl), stir for 1 h at 2-8°C.
- Centrifugation at 10E4 x g.
- Resuspend pellet in low salt buffer (e.g. 50 mM NaCl), stir for 30 min at 2-8°C.
- Centrifugation at 10E4 x g: pellet contains raw cytoskeletal fraction.
- Transfer from a low concentration acrylamide gel (e.g. 8%).
- Reduce the methanol concentration of the transfer buffer (limit is 50% of the routine concentration 20% methanol).
- Use the wet transfer method and transfer at least 4 h (or better overnight) at 100-200 mA.
- In order to prevent the accumulation of gas bubbles, stir the transfer buffer continuously during the transfer (e.g. with a magnetic stirrer).
- If possible use a cytoskeletal preparation of your material to be analyzed (in the case of cultured cells additionally DNase treatment) rather than whole cell lysate.
- As a general rule we would like to recommend loading that much protein into one gel lane that the intermediate filament polypeptides (in the 40-60 kD range) can be readily detected in a control gel stained with Coomassie blue.
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