Disease related

anti-Double strand DNA mouse monoclonal, AE-2, purified

Cat. No: 691529

Available, delivery time 1-7 days

$425.00*

Key Features
  • Mouse monoclonal
  • Suitable for FACS, ICC/IF and IHC
  • Reacts with all eukaryotes
  • Isotype: IgG3 kappa
Product description
Quantity 1 ml (100 µg/ml)
Antibody Type Monoclonal
Host Mouse
Isotype IgG3 kappa
Conjugate Unconjugated
Application FACS, ICC/IF, IHC
Purification Affinity chromatography
Reactivity All eukaryotes
Storage 2-8°C
Intended use Research use only
Clone AE-2
Immunogen Nuclei of Raji Burkitt’s lymphoma cells
Concentration 100 µg/ml
Formulation PBS with 0.02% sodium azide
Applications
Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) 1:50-1:100 (1-2 µg/ml)
Immunohistochemistry (IHC) - frozen 1:50-1:100 (1-2 µg/ml)
Immunohistochemistry (IHC) - paraffin 1:50-1:100 (1-2 µg/ml; microwave treatment in 4 N HCl recommended)
Flow Cytometry (FACS) 1-2 µg/million cells in 0.1 ml, fix cells in 4% PFA for 10 min at 4°C, permeabilize with 0.2% saponin or digitonin for 15 min at 4°C
Background

AE-2 recognizes double stranded DNA expressed in the nucleus of eukaryotes.

Positive control: human tonsil.

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