anti-Drebrin guinea pig polyclonal, serum
Species/Reactant: Mus musculus (House mouse)
Applications: Western Blotting
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- Guinea pig polyclonal
- Suitable for ICC/IF, IHC, IP and WB
- Reacts with bovine, human, mouse and rat
|Application||ICC/IF, IHC, IP, WB|
|Reactivity||Bovine, Human, Mouse, Rat|
|Storage||Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles|
|Intended use||Research use only|
|Immunogen||Synthetic human peptide (aa 254-272) coupled to KLH|
|Formulation||Contains 0.09% sodium azide|
|UniprotID||A1L5A7 (Bovine),Q16643 (Human),Q9QXS6 (Mouse),Q07266 (Rat)|
|Synonym||Drebrin, Developmentally-regulated brain protein, DBN1, D0S117E|
|Note||Centrifuge prior to opening|
|Tested applications||Tested dilutions|
|Immunocytochemistry (ICC)/ Immunofluorescence (IF)||Assay dependent|
|Immunohistochemistry (IHC) - frozen||1:100|
|Immunohistochemistry (IHC) - paraffin||1:100 (microwave treatment recommended)|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||Assay dependent|
The antiserum specifically reacts with drebrin, a widespread actin-associating protein of 70 kD (SDS-PAGE mobility shows Mr 120,000). Drebrin has originally been described only in neuronal cells, but has been recently found also in the actin-rich lamellipodia and filopodia of motile cells. It is enriched at junctional plaques, defining a specific microfilament anchorage system in polar epithelial cells.
Positive staining was found in many tissues, including diverse epithelia and carcinoma (e.g. in epidermis basal cells are positive, whereas overlying cells are negative; also positive: regenerating epithelium during wound healing; basal cell carcinoma; skin melanoma), specific types of endothelia and smooth muscle; especially prominent in the mesangial cells of renal glomeruli and in the Sertoli cells of testis.
Also positive: epithelia of hair follicles and eccrine sweat glands. Consistently negative were, however, hepatocytes and cross-striated muscle.
Positive cultured cell lines: PLC, U333, HUVEC, HaCat, HeLa, MCF-7, SV-80, B1; B16 (mouse melanoma cells)
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- Ascites fluid
- Mouse monoclonal
- Suitable for ICC/IF, IHC frozen and WB
- Reacts with bovine, dog, human, mouse, rat
- Isotype: IgG1