Frequently used
Diagnostic marker

anti-EP-CAM mouse monoclonal, HEA125, liquid, purified

Liquid

Liquid

Lyophilized

Prediluted

Cat. No: 690004

Available, delivery time 1-7 days

$425.00*

Key Features
  • Purified, liquid
  • Mouse monoclonal
  • Suitable for ICC/IF, IHC and WB
  • Reacts with human
  • Isotype: IgG1
Product description
Quantity 1 ml (50 µg/ml)
Antibody Type Monoclonal
Host Mouse
Isotype IgG1
Conjugate Unconjugated
Application FACS, ICC/IF, IHC, WB
Purification Affinity chromatography
Reactivity Human
No reactivity Mouse
Storage Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use Research use only
Clone HEA125
Immunogen HT-29 colon carcinoma cell line
Concentration 50 µg/ml
Formulation 0.09% sodium azide, 0.5% BSA in PBS buffer, pH 7.4
Synonym Human Epithelium Antigen, HEA, 17-1A, EPG34, CD326, TACSTD1
Applications
Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) Assay dependent
Immunohistochemistry (IHC) - frozen 1:10-1:50 (1-5 µg/ml)
Immunohistochemistry (IHC) - paraffin 1:10-1:50 (1-5 µg/ml; protease treatment recommended)
Flow Cytometry (FACS) Assay dependent
Western Blot (WB) Assay dependent (non-reducing conditions)
Background

The Ep-CAM (HEA125) antibody recognizes the epithelial cell adhesion molecule Ep-CAM (also described as 17-1A antigen, EPG34, CD326, and TACSTD1). This antigen is widely expressed on cells of epithelial origin and tumors derived therefrom. HEA125 represents an excellent marker to discriminate epithelial from mesothelial structures. The antigen has been detected in all carcinoma types tested (18 different origins). A subset of squamous cell carcinoma is negative.

The antibody can be used as alternative to the anti-EpCAM antibody clone BerEp4.

Polypeptide reacting: Mr 40,000 human epithelium-specific cell surface glycoprotein (Ep-CAM).

Reactivity on cultured cell lines: all carcinoma cell lines tested so far; particularly strong reaction with colon carcinoma cell lines.

References/Publications (7)
Publication
Species
Application
Downloads
File
Category
Size
Filetype
Category Protocol
Size 924.86 KB
Filetype pdf
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anti-EP-CAM mouse monoclonal, VU-1D9, ascites fluid
Cat. No: 16114
  • Mouse monoclonal
  • Suitable for ICC/IF and IHC on frozen and paraffin sections
  • Reacts with human

$326.00*