Epitope mapped
Diagnostic marker
anti-EP-CAM mouse monoclonal, VU-1D9, ascites fluid
Western blot analysis of human HT29 cell lysate with anti-Ep-CAM antibody. Western blot analysis was performed on 20 µg of HT29 lysate. Cells were lysed with RIPA buffer. The PVDF membrane was blocked with 5% dry milk in PBST (PBS + 0.1% Tween 20) for 1 h at RT. The primary antibodyanti-EP-CAM mouse monoclonal, VU-1D9 (Cat. No. 16114) was diluted in blocking buffer (1:5.000) and incubated for 1 h at RT. The secondary antibody anti-mouse IgG goat polyclonal, HRP conjugate was also diluted in blocking buffer (antibody concentration 0.2 µg/ml) and incubated for 1 h at RT. The bands were visualized by chemiluminescent detection using PierceTM ECL Western Blotting Substrate.
View more
Western blot analysis of human HT29 cell lysate with anti-Ep-CAM antibody. Western blot analysis was performed on 20 µg of HT29 lysate. Cells were lysed with RIPA buffer. The PVDF membrane was blocked with 5% dry milk in PBST (PBS + 0.1% Tween 20) for 1 h at RT. The primary antibodyanti-EP-CAM mouse monoclonal, VU-1D9 (Cat. No. 16114) was diluted in blocking buffer (1:5.000) and incubated for 1 h at RT. The secondary antibody anti-mouse IgG goat polyclonal, HRP conjugate was also diluted in blocking buffer (antibody concentration 0.2 µg/ml) and incubated for 1 h at RT. The bands were visualized by chemiluminescent detection using PierceTM ECL Western Blotting Substrate.
View more
Key Features
- Mouse monoclonal
- Suitable for WB, ICC/IF and IHC
- Reacts with human
Product description
| Quantity | 1 ml |
|---|---|
| Antibody Type | Monoclonal |
| Host | Mouse |
| Isotype | IgG1 |
| Conjugate | Unconjugated |
| Application | ICC/IF, IHC, WB |
| Purification | Ascites |
| Reactivity | Human |
| Storage | Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles |
| Intended use | Research use only |
| Clone | VU-1D9 |
| Immunogen | Isolated from small cell lung carcinoma-derived cell line (NC1-H69) |
| Formulation | Contains 0.09% sodium azide |
| Note | Centrifuge prior to opening |
Applications
| Tested applications | Tested dilutions |
|---|---|
| Immunocytochemistry (ICC)/ Immunofluorescence (IF) | Assay dependent |
| Immunohistochemistry (IHC) - frozen | 1:10-1:20 |
| Immunohistochemistry (IHC) - paraffin | 1:10-1:20 (microwave treatment recommended) |
| Western Blot (WB) | 1:200-1:5.000 |
Background
Ep-CAM (homophylic cell-cell adhesion molecule, also named ESA, EGP40, 17-1A antigen, KSA, GA7333-2) is a 40 kD epithelial protein expressed on the baso-lateral cell surface of many epithelial tissues. It is a transmembrane glycoprotein with 3 potential glycosyla-tion sites. The extracellular domain has a cysteine-rich repeat and a small domain with homology to nidogen. Antibody stains most epithelial cells and carcinoma derived therefrom.
Positive control: breast carcinoma.
Downloads
Q & A's
There aren't any asked questions yet.
Customer Reviews
Login
FAQs
The concentration of unpurified supernatant, ascites, unpurified guinea pig serum and unpurified rabbit serum is not determined.
The concentration of purified antibodies is mentioned on the datasheet.
For prediluted antibodies the concentration may vary from lot to lot. The concentration of these antibodies is not mentioned on the datasheet and can be requested at support@progen.com.
The concentration of purified antibodies is mentioned on the datasheet.
For prediluted antibodies the concentration may vary from lot to lot. The concentration of these antibodies is not mentioned on the datasheet and can be requested at support@progen.com.
Most of our purified mouse antibodies contain 0.5% BSA as stabilizer. If BSA was added to the antibody solution, it is stated in the datasheet.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.
Lyophilized antibodies can be stored at 2-8°C until expiration.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.
The expiration date of our antibodies is indicated on the product label.
Most of our antibodies contain 0.09% sodium azide as preservative. If a preservative is added, it is mentioned in the datasheet.
The optimal antibody dilution for your specific protocol and application needs to be titrated in your lab with your equipment and sample. The optimal dilution may vary between protocols and samples. A good dilution for starting the titration is the dilution mentioned in the datasheet. If the sample needs a specific treatment (eg. Antigen retrieval for IHC on FFPE sections) this should also be mentioned on the antibody datasheet.
PROGEN antibodies are shipped at ambient temperature. The antibodies are stable at ambient temperature for the shipment period. Please store the antibodies as indicated in the datasheet upon arrival.
Related Products
anti-EP-CAM mouse monoclonal, HEA125, lyophilized, purified
$251.00*
Cat. No: 61004
Quantity:
50 µg
- Purified, lyophilized
- Mouse monoclonal
- Suitable for ICC/IF, IHC and WB
- Reacts with human
- Isotype: IgG1
Variants from $96.00*