anti-Fibronectin mouse monoclonal, 568.0, supernatant
- Mouse monoclonal
- Suitable for ICC/IF, IHC on frozen and paraffin sections and WB
- Reacts with human
|Application||ICC/IF, IHC, WB|
|Purification||Hybridoma cell culture supernatant|
|Storage||Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles|
|Intended use||Research use only|
|Immunogen||Cultivated human fibroblasts|
|Formulation||Contains 0.09% sodium azide|
|Synonym||Fibronectin, FN, Cold-insoluble globulin, CIG [Cleaved into: Anastellin; Ugl-Y1; Ugl-Y2; Ugl-Y3], FN1, FN|
|Note||Centrifuge prior to opening|
|Tested applications||Tested dilutions|
|Immunocytochemistry (ICC)/ Immunofluorescence (IF)||1:5-1:10|
|Immunohistochemistry (IHC) - frozen||1:5-1:10|
|Immunohistochemistry (IHC) - paraffin||1:5-1:10 (microwave treatment recommended)|
|Western Blot (WB)||Assay dependent|
Mab 568 reacts with the cell binding part of the fibronectin molecule. Fibronectin seems to play a key role in the tumor cell extracellular matrix during invasive tumor growth. Increasing expression of stromal fibronectin has been reported during tumor progression. In immunohistochemistry the antibody is reactive with an extracellular matrix glycoprotein in vessels and connective tissue.
Positive control: Human skin, ulcerating tissue
Q & A's
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The concentration of purified antibodies is mentioned on the datasheet.
For prediluted antibodies the concentration may vary from lot to lot. The concentration of these antibodies is not mentioned on the datasheet and can be requested at firstname.lastname@example.org.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.