Disease related

anti-Glial Fibrillary Acidic Protein mouse monoclonal, GF 12.24, lyophilized, purified

Prediluted

Lyophilized

Cat. No: 61011

Available, delivery time 1-7 days

$241.00*

Key Features
  • Purified, lyophilized
  • Mouse monoclonal
  • Suitable for ICC/IF, IHC and WB
  • Reacts with bovine, human, mouse, rat
  • Isotype: IgG2a
Product description
Quantity 50 µg
Antibody Type Monoclonal
Host Mouse
Isotype IgG2a
Conjugate Unconjugated
Application ICC/IF, IHC, WB
Purification Affinity chromatography
Reactivity Bovine, Human, Mouse, Rat
Storage before reconstitution 2-8°C until indicated expiry date
Storage after reconstitution Up to 3 months at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use Research use only
Clone GF 12.24
Immunogen Intermediate filament cytoskeleton from cultured human glioma cells
Concentration 50 µg/ml after reconstitution with 1 ml dist. water
Formulation Lyophilized; reconstitute in 1 ml dist. water (final solution contains 0.09% sodium azide, 0.5% BSA in PBS buffer, pH 7.4)
UniprotID Q28115 (Bovine),P14136 (Human),P03995 (Mouse),P47819 (Rat)
Synonym Glial fibrillary acidic protein, GFAP, Glial Filament Protein, GFP
Applications
Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) Assay dependent
Immunohistochemistry (IHC) - frozen 1:10
Immunohistochemistry (IHC) - paraffin 1:10 (microwave treatment recommended)
Western Blot (WB) Assay dependent
Background

GF 12.24 represents an excellent marker for cell typing. Suitable for prenatal diagnosis of neural tube defects. Polypeptide reacting: Mr 50,000 glial filament protein GFAP (Glial Fibrillary Acidic Protein, Glial Filament Protein). Tumors specifically reacting: astrocytomas, gangliomas, medulloblastomas, mixed gliomas, certain ependymomas, certain teratomas.

Reactivity on cultured cell lines: human U 333 CG/343MG

References/Publications (1)
Publication
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