anti-Glicentin/Glucagon rabbit polyclonal, serum

Cat. No: 16034

Available, delivery time 1-7 days

$514.00*

Key Features
  • Rabbit polyclonal
  • Suitable for ICC/IF and IHC on frozen and paraffin sections
  • Reacts with hamster, human, pig, rat
Product description
Quantity 50 µl (lyoph.)
Antibody Type Polyclonal
Host Rabbit
Conjugate Unconjugated
Application ICC/IF, IHC
Purification Undiluted antiserum
Reactivity Hamster, Human, Pig, Rat
Storage before reconstitution 2-8°C until indicated expiry date
Storage after reconstitution Up to 3 months at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use Research use only
Immunogen Porcine pancreatic glucagon (Novo, Denmark), conjugated to BSA
Formulation Lyophilized; reconstitute in 100 µl dist. water
UniprotID P01275 (Human),P01274 (Pig),P05110 (Guinea pig),P06883 (Rat)
Synonym Pro-glucagon [Cleaved into: Glicentin; Glicentin-related polypeptide, GRPP; Oxyntomodulin, OXM, OXY; Glucagon; Glucagon-like peptide 1, GLP-1, Incretin hormone; Glucagon-like peptide 1(7-37, GLP-1(7-37; Glucagon-like peptide 1(7-36, GLP-1(7-36; Glucagon-like peptide 2, GLP-2], GCG
Applications
Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) 1:1,000-1:3,000
Immunohistochemistry (IHC) - frozen 1:1,000-1:3,000
Immunohistochemistry (IHC) - paraffin 1:1,000-1:3,000 (microwave treatment recommended)
Background

Glicentin contains the glucagon sequence and is produced in a prominent population of endocrine cells in the distal intestine as well as in pancreatic glucagon cells and in the nerves in the brain. Serum levels of glicentin are elevated after food uptake and in certain clinical conditions, e.g. after resections of the intestine. The functional role of glicentin is largely unknown. Glicentin occurs in endocrine tumors arising in the distal intestine (rectal carcinoids) and in pancreatic islet cell tumors.
Absorption with 10-100 ug glucagon and glicentin per ml diluted antiserum abolishes the staining, while secretin, GIP and VIP do not.

Positive control: formalin-fixed paraffin sections of pig pancreas.

References/Publications (2)
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FAQs
The concentration of unpurified supernatant, ascites, unpurified guinea pig serum and unpurified rabbit serum is not determined.
The concentration of purified antibodies is mentioned on the datasheet.
For prediluted antibodies the concentration may vary from lot to lot. The concentration of these antibodies is not mentioned on the datasheet and can be requested at support@progen.com.

Most of our purified mouse antibodies contain 0.5% BSA as stabilizer. If BSA was added to the antibody solution, it is stated in the datasheet.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.

Lyophilized antibodies can be stored at 2-8°C until expiration.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.

The expiration date of our antibodies is indicated on the product label.
Most of our antibodies contain 0.09% sodium azide as preservative. If a preservative is added, it is mentioned in the datasheet.
The optimal antibody dilution for your specific protocol and application needs to be titrated in your lab with your equipment and sample. The optimal dilution may vary between protocols and samples. A good dilution for starting the titration is the dilution mentioned in the datasheet. If the sample needs a specific treatment (eg. Antigen retrieval for IHC on FFPE sections) this should also be mentioned on the antibody datasheet. 
PROGEN antibodies are shipped at ambient temperature. The antibodies are stable at ambient temperature for the shipment period. Please store the antibodies as indicated in the datasheet upon arrival.
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