anti-Glutamic Acid Decarboxylase (GAD 65) rabbit polyclonal, serum
- Rabbit polyclonal
- Suitable for IHC on frozen and paraffin sections
- Reacts with rat
|Quantity||100 µl (lyoph.)|
|Storage before reconstitution||2-8°C until indicated expiry date|
|Storage after reconstitution||Up to 3 months at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles|
|Intended use||Research use only|
|Immunogen||Synthetic GAD 65 (520-534), conjugated to BSA|
|Formulation||Lyophilized; reconstitute in 100 µl dist. water|
|Tested applications||Tested dilutions|
|Immunohistochemistry (IHC) - frozen||1:100-1:300|
|Immunohistochemistry (IHC) - paraffin||1:100-1:300 (microwave treatment recommended)|
Glutamic acid decarboxylase (GAD) is the GABA-synthesizing enzyme as well as a major autoantigen in insulin-dependent diabetes mellitus (DDM). GAD occurs in at least two isoforms, GAD 65 and GAD 67. The antibody was raised by immunizing with a synthetic peptide from the C-terminus of GAD 65. Absorption with 10-100 ug immunogen per ml diluted antiserum abolishes the staining.
Stefanini-fixed frozen sections of rat pancreas.
Q & A's
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The concentration of purified antibodies is mentioned on the datasheet.
For prediluted antibodies the concentration may vary from lot to lot. The concentration of these antibodies is not mentioned on the datasheet and can be requested at firstname.lastname@example.org.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.