Frequently used

anti-HA-tag mouse monoclonal, 12CA5, lyophilized, purified

Lyophilized

Lyophilized

Cat. No: 9100HA

Available, delivery time 1-7 days

$109.00*

Key Features
  • Purified, lyophilized
  • Mouse monoclonal
  • Suitable for WB
  • Reacts with HA-tag
  • Isotype: IgG2b
Product description
Quantity 25 µg
Antibody Type Monoclonal
Host Mouse
Isotype IgG2b
Conjugate Unconjugated
Application WB
Purification Affinity chromatography
Reactivity HA
Storage before reconstitution 2-8°C until indicated expiry date
Storage after reconstitution -20°C (avoid freeze/thaw cycles)
Intended use Research use only
Clone 12CA5
Immunogen 9 amino acids from the human influenza virus hemagglutinin protein
Concentration 0.25 mg/ml after reconstitution with 100 µl PBS
Formulation Lyophilized; reconstitute in 100 µl sterile PBS, pH 7.4
Applications
Tested applications Tested dilutions
Western Blot (WB) 1:5,000-1:10,000 (0.05-0.025 µg/ml)
Background
The monoclonal 12CA5 antibody recognizes the HA-tag (hemagglutinin tag). The HA-tag is commonly added to recombinant proteins and can be used for detection or purification of the tagged protein.
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FAQs
The concentration of unpurified supernatant, ascites, unpurified guinea pig serum and unpurified rabbit serum is not determined.
The concentration of purified antibodies is mentioned on the datasheet.
For prediluted antibodies the concentration may vary from lot to lot. The concentration of these antibodies is not mentioned on the datasheet and can be requested at support@progen.com.

Most of our purified mouse antibodies contain 0.5% BSA as stabilizer. If BSA was added to the antibody solution, it is stated in the datasheet.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.

Lyophilized antibodies can be stored at 2-8°C until expiration.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.

Most of our antibodies contain 0.09% sodium azide as preservative. If a preservative is added, it is mentioned in the datasheet.
The optimal antibody dilution for your specific protocol and application needs to be titrated in your lab with your equipment and sample. The optimal dilution may vary between protocols and samples. A good dilution for starting the titration is the dilution mentioned in the datasheet. If the sample needs a specific treatment (eg. Antigen retrieval for IHC on FFPE sections) this should also be mentioned on the antibody datasheet. 
PROGEN antibodies are shipped at ambient temperature. The antibodies are stable at ambient temperature for the shipment period. Please store the antibodies as indicated in the datasheet upon arrival.
anti-GFP-tag mouse monoclonal, F56-6A1.2.3, lyophilized, purified
Cat. No: 910GFP
Quantity: 25 µg
  • Purified, lyophilized
  • Mouse monoclonal
  • Suitable for ICC and WB
  • Reacts with GFP-tag
  • Isotype: IgG2b

$109.00*
anti-DDDDK-tag mouse monoclonal, AP1501, lyophilized, purified
Cat. No: 910DDK
Quantity: 25 µg
  • Purified, lyophilized
  • Mouse monoclonal
  • Suitable for ICC, IP and WB
  • Reacts with DDDDK-tag
  • Isotype: IgG2b

$109.00*
anti-Strep-tag mouse monoclonal, C23.21, lyophilized, purified
Cat. No: 910STR
Quantity: 25 µg
  • Purified, lyophilized
  • Mouse monoclonal
  • Suitable for WB
  • Reacts with Strep-tag
  • Isotype: IgG1

$109.00*
anti-c-myc-tag mouse monoclonal, 9E10, lyophilized, purified
Cat. No: 910MYC
Quantity: 25 µg
  • Purified, lyophilized
  • Mouse monoclonal
  • Suitable for ELISA, IP and WB
  • Reacts with c-myc-tag
  • Isotype: IgG1

$109.00*
anti-6-His-tag mouse monoclonal, 6His, lyophilized, purified
Cat. No: 910HIS
Quantity: 25 µg
  • Purified, lyophilized
  • Mouse monoclonal
  • Suitable for ICC, IP and WB
  • Reacts with 6-His-tag
  • Isotpye: IgG1

$109.00*
anti-GST-tag mouse monoclonal, F50-3D12.2, lyophilized, purified
Cat. No: 910GST
Quantity: 25 µg
  • Purified, lyophilized
  • Mouse monoclonal
  • Suitable for WB
  • Reacts with GST-tag
  • Isotype: IgG1

$109.00*
proflo His-tag quick test
Cat. No: PRLFHIS
  • Competitive immuno-chromatographic test
  • Detection of histidine-tagged recombinant proteins
  • Samples: cell lysates or purified protein preparations

$114.00*