Disease related

anti-HSP60 mouse monoclonal, HSP60-1, purified

Cat. No: 691697

Available, delivery time 1-7 days

$425.00*

Key Features
  • Mouse monoclonal
  • Suitable for FACS, ICC/IF, IHC and WB
  • Reacts with bovine, chicken, dog, hamster, human, monkey, pig, rabbit, rat and sheep
  • Isotype: IgG1 kappa
Product description
Quantity 1 ml (100 µg/ml)
Antibody Type Monoclonal
Host Mouse
Isotype IgG1 kappa
Conjugate Unconjugated
Application FACS, ICC/IF, IHC, WB
Purification Affinity chromatography
Reactivity Bovine, Chicken, Dog, Hamster, Human, Monkey, Pig, Rabbit, Rat, Sheep
Storage 2-8°C
Intended use Research use only
Clone HSP60-1
Immunogen Recombinant human HSPD1
Concentration 100 µg/ml
Formulation PBS with 0.02% sodium azide
UniprotID P31081 (Bovine), Q5ZL72 (Chicken), P10809 (Human), A0A287ATN8 (Pig), P63039 (Rat), A0A6P7DQC9 (Sheep)
Synonym 60 kDa heat shock protein, mitochondrial, EC 5.6.1.7, 60 kDa chaperonin, Chaperonin 60, CPN60, Heat shock protein 60, HSP-60, Hsp60, HuCHA60, Mitochondrial matrix protein P1, P60 lymphocyte protein, HSPD1, HSP60
Applications
Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) 1:100-1:200 (0.5-1.0 µg/ml)
Immunohistochemistry (IHC) - frozen 1:50-1:100 (1-2 µg/ml)
Immunohistochemistry (IHC) - paraffin 1:50-1:100 (1-2 µg/ml; microwave treatment in 10 mM citrate buffer pH 6.0 recommended)
Flow Cytometry (FACS) 0.5-1.0 µg/million cells in 0.1 ml
Western Blot (WB) 1:50-1:100 (1-2 µg/ml)
Background

HSP60-1 reacts with Heat Shock Protein 60 or HSPD1, found in mitochondria. A wide variety of environmental and pathophysiological stressful conditions trigger the synthesis of a family of proteins known as heat shock proteins (HSP's), more appropriately called as Stress Response Proteins (SRP's). HSP60 is a potential antigen in a number of autoimmune diseases. In human arthritis and in experimentally induced arthritis in animals, disease development coincides with the development of immune reactivity directed against not only bacterial HSP60, but also against its mammalian.

Positive control: HeLa or HepG2 cells. Synovial biopsies from patients with juvenile chronic arthritis. Synovial lining layer is strongly positive for hsp60. Carcinomas.

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