anti-Helicobacter pylori mouse monoclonal, EBS-I-041, purified
- Mouse monoclonal
- Suitable for ELISA, FACS, ICC/IF and IHC
- Reacts with H. pylori
- Isotype: IgG1 kappa
Product description
| Quantity | 1 ml (100 µg/ml) |
|---|---|
| Antibody Type | Monoclonal |
| Host | Mouse |
| Isotype | IgG1 kappa |
| Conjugate | Unconjugated |
| Application | ELISA, FACS, ICC/IF, IHC |
| Purification | Affinity chromatography |
| Reactivity | H. pylori |
| Storage | 2-8°C |
| Intended use | Research use only |
| Clone | EBS-I-041 |
| Immunogen | Sonicated Helicobacter pylori |
| Concentration | 100 µg/ml |
| Formulation | PBS with 0.02% sodium azide |
Applications
| Tested applications | Tested dilutions |
|---|---|
| Immunocytochemistry (ICC)/ Immunofluorescence (IF) | 1:100-1:200 (0.5-1.0 µg/ml) |
| Immunohistochemistry (IHC) - frozen | 1:50-1:100 (1-2 µg/ml) |
| ELISA | Assay dependent |
| Flow Cytometry (FACS) | 0.5-1.0 µg/million cells in 0.1 ml |
Background
Over half of the human population is infected with H. pylori. It is usually acquired in childhood and, when left untreated, generally persists for the host's life. Usually infection leads to no more than superficial or asymptomatic gastritis, however in some cases it is implicated in the development peptic ulcers, gastric cancer and low-grade lymphoma of mucosa associated lymphoid tissue (MALT). It is further associated with duodenal ulcers.
Positive control: H. pylori infected stomach biopsy.
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FAQs
The concentration of purified antibodies is mentioned on the datasheet.
For prediluted antibodies the concentration may vary from lot to lot. The concentration of these antibodies is not mentioned on the datasheet and can be requested at support@progen.com.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.
- Supernatant and supernatant concentrate: This format contains hybridoma cell culture supernatant. The antibody is not purified and the antibody concentration is not determined. The antibody concentration may vary from lot to lot. Therefore we recommend to titrate the optimal concentration for the application used for each new lot.
- Lyophilized, purified: This format contains purified antibody in lyophilized form. The reconstitution of this antibody is described in the datasheet. The buffer composition after reconstitution is also mentioned on the datasheet.
- Liquid, purified: This format contains purified antibody in liquid format. The concentration is mentioned on the datasheet.
- Prediluted, purified: This format contains purified antibody in liquid format. Most antibodies in this format are diluted to be ready-to-use for IHC with standard tissue. But some antibodies of this format need further dilution for IHC. This is mentioned on the datasheet.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.
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