anti-Helospectin rabbit polyclonal, serum
- Rabbit polyclonal
- Suitable for IHC on frozen sections
- Reacts with cat, dog, mouse, rat
|Quantity||50 µl (lyoph.)|
|Reactivity||Cat, Dog, Mouse, Rat|
|Storage before reconstitution||2-8°C until indicated expiry date|
|Storage after reconstitution||Up to 3 months at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles|
|Intended use||Research use only|
|Immunogen||Synthetic lizard Helospectin I (#7148, Peninsula)|
|Formulation||Lyophilized; reconstitute in 100 µl dist. water|
|Tested applications||Tested dilutions|
|Immunohistochemistry (IHC) - frozen||1:400-1:800|
The peptide was found in the salivary gland venom of the lizard Gila monster. It belongs to the VIP-secretin family of peptides and has many VIP-like actions. Helospectin-immunoreactivity has been demonstrated in the neurons of the gut.
Absorption with 10-100 ug helospectin per ml diluted antiserum abolishes the staining, while secretin, PHI and PACAP do not.
Cross-reacts with helodermin.
Positive control: Stefanini-fixed frozen sections of rat small intestine (1% carbodiimide added to the fixative).
Q & A's
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The concentration of purified antibodies is mentioned on the datasheet.
For prediluted antibodies the concentration may vary from lot to lot. The concentration of these antibodies is not mentioned on the datasheet and can be requested at email@example.com.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.