anti-Histidine Decarboxylase rabbit polyclonal, serum
- Rabbit polyclonal
- Suitable for ICC/IF, IHC on frozen and paraffin sections and WB
- Reacts with dog, guinea pig, human, mouse, rat
|Quantity||50 µl (lyoph.)|
|Application||ICC/IF, IHC, WB|
|Reactivity||Dog, Guinea pig, Human, Mouse, Rat|
|Storage before reconstitution||2-8°C until indicated expiry date|
|Storage after reconstitution||Up to 3 months at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles|
|Intended use||Research use only|
|Immunogen||Recombinant histidine decarboxylase produced in E. coli|
|Formulation||Lyophilized; reconstitute in 100 µl dist. water|
|UniprotID||E2RMU1 (Dog, Canis familiaris),H0WB71 (Guinea pig),P19113 (Human),Q8CHP0 (Mouse),P16453 (Rat)|
|Synonym||Histidine decarboxylase, HDC, EC 220.127.116.11, HDC|
|Tested applications||Tested dilutions|
|Immunocytochemistry (ICC)/ Immunofluorescence (IF)||Assay dependent|
|Immunohistochemistry (IHC) - frozen||1:2,000-1:5,000|
|Immunohistochemistry (IHC) - paraffin||1:2,000-1:5,000 (microwave treatment recommended)|
|Western Blot (WB)||1:1,000-1:5,000|
Histidine decarboxylase (HDC) is the enzyme catalyzing the conversion of histidine into histamine. HDC can be found in the histamine secreting ECL cells of some species as well as in the mast cells. Absorption with 10-100 ug immunogen per ml diluted antiserum abolishesthe staining.
In Western blot the antiserum detects the 54 kDa and 73 kDa forms in addition to a 63 kDa form (rat stomach, see Dartsch et al., 1998).
Positive control: Stefanini-fixed frozen sections of rat fundus.
Dartsch, C., Chen, D. & Persson, L. Multiple forms of rat stomach histidine decarboxylase may reflect posttranslational activation of the enzyme. Regul. Pept. 77, 33-41 (1998).
Q & A's
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The concentration of purified antibodies is mentioned on the datasheet.
For prediluted antibodies the concentration may vary from lot to lot. The concentration of these antibodies is not mentioned on the datasheet and can be requested at firstname.lastname@example.org.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.