Disease related

anti-Histone H1 mouse monoclonal, AE-4, purified

Cat. No: 691531

Available, delivery time 1-7 days

$425.00*

Key Features
  • Mouse monoclonal
  • Suitable for FACS, ICC/IF and IHC
  • Reacts with human, mouse and rat
  • Isotype: IgG2a kappa
Product description
Quantity 1 ml (100 µg/ml)
Antibody Type Monoclonal
Host Mouse
Isotype IgG2a kappa
Conjugate Unconjugated
Application FACS, ICC/IF, IHC
Purification Affinity chromatography
Reactivity Human, Mouse, Rat
Storage 2-8°C
Intended use Research use only
Clone AE-4
Immunogen Human leukemia biopsy cells
Concentration 100 µg/ml
Formulation PBS with 0.02% sodium azide
UniprotID P07305 (Human), P10922 (Mouse), P43278 (Rat)
Synonym Histone H1.0, Histone H1', Histone H1(0 [Cleaved into: Histone H1.0, N-terminally processed], H1-0, H1F0, H1FV
Applications
Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) 1:100-1:200 (0.5-1.0 µg/ml)
Immunohistochemistry (IHC) - frozen 1:50-1:100 (1-2 µg/ml)
Immunohistochemistry (IHC) - paraffin 1:50-1:100 (1-2 µg/ml; microwave treatment in 10 mM citrate buffer pH 6.0 recommended)
Flow Cytometry (FACS) 1-2 µg/million cells in 0.1 ml, fix cells in 4% PFA for 10 min at 4°C, permeabilize with 0.2% saponin or digitonin for 15 min at 4°C
Background

AE-4 recognizes histone H1 in the nuclei of animal and plant cells. It produces a speckled nuclear staining pattern in normal and malignant cells.

Positive control: HeLa, A-431, LNCap or Jurkat cells. Breast carcinoma or tonsil.

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