anti-Insulin/Proinsulin guinea pig polyclonal, serum
- Guinea pig polyclonal
- Suitable for ICC/IF and IHC on frozen and paraffin sections
- Reacts with hamster, human, pig and rat
|Quantity||50 µl (lyoph.)|
|Reactivity||Hamster, Human, Pig, Rat|
|Storage before reconstitution||2-8°C until indicated expiry date|
|Storage after reconstitution||Up to 3 months at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles|
|Intended use||Research use only|
|Immunogen||Synthetic human proinsulin|
|Formulation||Lyophilized; reconstitute in 100 µl dist. water|
|Tested applications||Tested dilutions|
|Immunocytochemistry (ICC)/ Immunofluorescence (IF)||1:1,000-1:2,000|
|Immunohistochemistry (IHC) - frozen||1:3,000-1:5,000 (PAP)|
|Immunohistochemistry (IHC) - paraffin||1:3,000-1:5,000 (PAP)|
Insulin is produced by the B-cells of the pancreatic islets and by insulin-producing islet cell tumors.
Absorption with 10-100 ug proinsulin per ml diluted antiserum inactivates the antiserum, while C-peptide and insulin only partly reduce staining.
Positive control: formalin-fixed paraffin sections of human pancreas.
Q & A's
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The concentration of purified antibodies is mentioned on the datasheet.
For prediluted antibodies the concentration may vary from lot to lot. The concentration of these antibodies is not mentioned on the datasheet and can be requested at email@example.com.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.
- Methanol/ acetone fixation: Immerse slide in precooled (-20°C) methanol for 5 min, immerse in precooled (-20°C) acetone for 30-60 sec, let specimen air dry before antibody incubation.
- Methanol/ acetone fixation plus detergent permeabilization: After methanol/ acetone fixation and air-drying dip slide either in a solution containing 0.1-0.2% Triton X-100 in PBS or in 0.1% saponin in PBS for 1-5 min at room temperature (enhances accessibility of many cytoskeletal antigens).
- Air-drying of the section.
- Block with the serum of the species in which the secondary antibody was raised for 30 min.
- Incubation with 1st antibody 1 h at RT in moist chamber.
- Wash 3x with PBS.
- Incubation with appropriate fluorescent secondary antibody, 30-60 min at RT.
- Wash 3x with PBS.
- Immerse shortly into ethanol.
- Let air dry.
- Cover with mounting medium.
In guinea pigs the antibody concentration in serum varies from 10 to 20 mg/ml; specific antibodies represent normally about 0.1-1% of total IgG. Total protein concentration varies from 40 to 65 mg/ml, with the main constituent (about 60%) being albumin.