Epitope mapped

anti-Interferon alpha 2 mouse monoclonal, N27, purified

Cat. No: 691711

Available, delivery time 1-7 days


Key Features
  • Mouse monoclonal
  • Suitable for ELISA, IHC and WB
  • Reacts with human
  • Isotype: IgG1 kappa
Product description
Quantity 1 ml (100 µg/ml)
Antibody Type Monoclonal
Host Mouse
Isotype IgG1 kappa
Conjugate Unconjugated
Application ELISA, IHC, WB
Purification Affinity chromatography
Reactivity Human
Storage 2-8°C
Intended use Research use only
Clone N27
Immunogen E. coli derived recombinant human IFN alpha 2c
Concentration 100 µg/ml
Formulation PBS with 0.02% sodium azide
UniprotID P01563 (Human)
Synonym Interferon alpha-2, IFN-alpha-2, Interferon alpha-A, LeIF A, IFNA2, IFNA2A, IFNA2B, IFNA2C
Tested applications Tested dilutions
Immunohistochemistry (IHC) - frozen 1:50-1:100 (1-2 µg/ml)
ELISA Assay dependent
Western Blot (WB) 1:50-1:100 (1-2 µg/ml)

The alpha interferons are involved in virus resistance in target cells for these viruses. They are known to block cell proliferation and to regulate MHC class I antigen expression. The IFN alpha family has over 20 genes and pseudogenes in two families (I and II), one with a mature length of 166aa and one of 172aa. Cells producing IFN alpha are lymphocytes, monocytes, macrophages and cell lines such as Namalwa and KGI. Bioassays for IFN alpha include cytopathic effect blocking, by viruses such as VSV, SFV and BMCV, on their target cells. A number of receptors for IFN alpha are now known and seem to be expressed on most cell types. N27 is specific for human IFN alpha 2 and does not cross react with human IFN alpha 1. N27 reacts with linear peptide 43aa-53aa, placing the epitope outside the immunodominant regions I and II.

Positive control: human IFN alpha 2, Namalwa and KGI cells.

Pair: N39

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