anti-Keratin K12 guinea pig polyclonal, serum

Cat. No: GP-K12

Available, delivery time 1-7 days

$311.00*

Key Features
  • Guinea pig polyclonal
  • Suitable for IHC
  • Reacts with human, mouse and pig
Product description
Quantity 100 µl
Antibody Type Polyclonal
Host Guinea pig
Conjugate Unconjugated
Application IHC
Purification Stabilized antiserum
Reactivity Human, Mouse, Pig
Storage Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use Research use only
Immunogen Synthetic peptide of human keratin K12 (formerly also designated cytokeratin 12; DLS NNT MSL SVR T-C), coupled to KLH
Formulation Contains 0.09% sodium azide and 0.5% BSA
UniprotID Q99456 (Human),Q64291 (Mouse)
Synonym Keratin, type I cytoskeletal 12, Cytokeratin-12, CK-12, Keratin-12, K12, KRT12
Note Centrifuge prior to opening
Applications
Tested applications Tested dilutions
Immunohistochemistry (IHC) - frozen 1:100-1:200
Immunohistochemistry (IHC) - paraffin 1:100-1:200 (microwave treatment recommended)
Background

Useful as a marker for corneal type differentiation (positive for epithelial cells of cornea) and for studying corneal epithelial stem cells.
Reactive polypeptide: MW 55,000 (pI 4.9) intermediate filament polypeptide, keratin K12, detected by immunohistochemistry in the cornea only.
Useful as a marker for corneal type differentiation (positive for epithelial cells of cornea) and for studying corneal epithelial stem cells.
Positive control: human cornea.

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FAQs
Most of our purified mouse antibodies contain 0.5% BSA as stabilizer. If BSA was added to the antibody solution, it is stated in the datasheet.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.

Lyophilized antibodies can be stored at 2-8°C until expiration.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.

The expiration date of our antibodies is indicated on the product label.
  1. Methanol/ acetone fixation: Immerse slide in precooled (-20°C) methanol for 5 min, immerse in precooled (-20°C) acetone for 30-60 sec, let specimen air dry before antibody incubation.
  2. Methanol/ acetone fixation plus detergent permeabilization: After methanol/ acetone fixation and air-drying dip slide either in a solution containing 0.1-0.2% Triton X-100 in PBS or in 0.1% saponin in PBS for 1-5 min at room temperature (enhances accessibility of many cytoskeletal antigens).

  • Air-drying of the section.
  • Block with the serum of the species in which the secondary antibody was raised for 30 min.
  • Incubation with 1st antibody 1 h at RT in moist chamber.
  • Wash 3x with PBS.
  • Incubation with appropriate fluorescent secondary antibody, 30-60 min at RT.
  • Wash 3x with PBS.
  • Immerse shortly into ethanol.
  • Let air dry.
  • Cover with mounting medium.

Most of our antibodies contain 0.09% sodium azide as preservative. If a preservative is added, it is mentioned in the datasheet.
The optimal antibody dilution for your specific protocol and application needs to be titrated in your lab with your equipment and sample. The optimal dilution may vary between protocols and samples. A good dilution for starting the titration is the dilution mentioned in the datasheet. If the sample needs a specific treatment (eg. Antigen retrieval for IHC on FFPE sections) this should also be mentioned on the antibody datasheet. 
PROGEN antibodies are shipped at ambient temperature. The antibodies are stable at ambient temperature for the shipment period. Please store the antibodies as indicated in the datasheet upon arrival.

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