Highly published

anti-Keratin K14 guinea pig polyclonal, serum

Cat. No: GP-CK14

Prices excl. tax

Available, delivery time 1-7 days

$360.00*

Datasheet

Key Features

Stabilized antiserum
Guinea pig polyclonal
Suitable for IHC and WB
Reacts with human, mouse

Product description

Quantity	100 µl
Antibody Type	Polyclonal
Host	Guinea pig
Conjugate	Unconjugated
Application	IHC, WB
Purification	Stabilized antiserum
Reactivity	Human, Mouse
Storage	Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use	Research use only
Immunogen	Peptide against human K14 C-VSTHEQVLRTKN (460-472)
Formulation	Contains 0.09% sodium azide and 0.5% BSA
UniprotID	P02533 (Human), Q61781 (Mouse)
Synonym	Keratin, type I cytoskeletal 14, Cytokeratin-14, CK-14, Keratin-14, K14, KRT14
Note	Centrifuge prior to opening

Applications

Tested applications	Tested dilutions
Immunohistochemistry (IHC) - frozen	1:100-1:200
Immunohistochemistry (IHC) - paraffin	1:50 (microwave treatment recommended)
Western Blot (WB)	1:5,000-1:10,000

Background

Reactive Polypeptide: acidic keratin K14 (Mr 50,000; formerly also designated cytokeratin 14), expressed in basal and first suprabasal layers of epidermis.
Tested cultured cell lines: BPH-1 (derived from non-neoplastic prostatic tissue).
Tumors specifically detected: the antiserum reacts with keratin K14, expressed in the basal cells of the larynx, esophagus, trachea, bladder, prostate, cervix, vagina, breast acini, skin and sweat glands. In several studies the correlation between the expression of keratin K14 in different types of carcinomas (putatively derived from basal epithelial cells) and prognosis has been discussed (see references).

References/Publications (17)

Publication

Species

Application

Publication Peter J Koch, Saiphone Webb, Jessica A Gugger, Maddison N Salois, Maranke I Koster. Differentiation of Human Induced Pluripotent Stem Cells into Keratinocytes., Curr Protoc 2, e408, (2022).

Species human

Application ICC-IF

Publication Allmeroth, K. et al. N1-acetylspermidine is a determinant of hair follicle stem cell fate. J. Cell Sci. 134, (2021).

Species mouse

Application IHC-IF (paraffin)

Publication Azazmeh, N. et al. Chronic expression of p16INK4a in the epidermis induces Wnt-mediated hyperplasia and promotes tumor initiation. Nat. Commun. 11, (2020).

Species mouse

Application IHC, IHC-IF

Publication Wu, L. et al. Anti-CD47 treatment enhances anti-tumor T-cell immunity and improves immunosuppressive environment in head and neck squamous cell carcinoma. Oncoimmunology. 7, e1397248 (2018)

Species mouse

Application IHC-IF (paraffin)

Publication Yosef, R. et al. Directed elimination of senescent cells by inhibition of BCL-W and BCL-XL. Nat. Commun. 7, (2016).

Species mouse

Application IHC (paraffin)

Publication Yang, C. et al. Plakophilin 1-deficient cells upregulate SPOCK1: implications for prostate cancer progression. Tumour Biol. 36, 9567–77 (2015).

Species human

Application WB,IHC (paraffin)

Publication Kist, R. et al. The Formation of Endoderm-Derived Taste Sensory Organs Requires a Pax9-Dependent Expansion of Embryonic Taste Bud Progenitor Cells. PLoS Genet. 10, (2014).

Species mouse

Application IHC (frozen)

Publication Cui, C.-Y. et al. Involvement of Wnt, Eda and Shh at defined stages of sweat gland development. Development 141, 3752–60 (2014).

Species mouse

Application IHC (paraffin)

Publication Tokarsky-Amiel, R. et al. Dynamics of Senescent Cell Formation and Retention Revealed by p14ARF Induction in the Epidermis. Cancer Res. 73, 2829–2839 (2013).

Species mouse

Application IHC (paraffin)

Publication Wallace, L., Roberts-Thompson, L. & Reichelt, J. Deletion of K1/K10 does not impair epidermal stratification but affects desmosomal structure and nuclear integrity. J. Cell Sci. 125, 1750–1758 (2012).

Species mouse

Application IHC (frozen)

Publication Cui, C.-Y. et al. Forkhead transcription factor FoxA1 regulates sweat secretion through Bestrophin 2 anion channel and Na-K-Cl cotransporter 1. Proc. Natl. Acad. Sci. United States Am. 109, 1199–1203 (2012).

Species mouse

Application IHC (paraffin)

Publication Langbein, L., Yoshida, H., Praetzel-Wunder, S., Parry, D. A. & Schweizer, J. The Keratins of the Human Beard Hair Medulla: The Riddle in the Middle. J. Invest. Dermatol. 130, 55–73 (2010).

Species human

Application IHC (frozen)

Publication Jennemann, R. et al. Integrity and Barrier Function of the Epidermis Critically Depend on Glucosylceramide Synthesis. J. Biol. Chem. 282, 3083–3094 (2006).

Species mouse

Application IHC (paraffin)

Publication Peitsch, W. K. et al. Drebrin, an Actin-Binding, Cell-Type Characteristic Protein: Induction and Localization in Epithelial Skin Tumors and Cultured Keratinocytes. J. Invest. Dermatol. 125, 761–774 (2005).

Species human

Application IHC (frozen)

Publication Langbein, L. et al. Characterization of a Novel Human Type II Epithelial Keratin K1b, Specifically Expressed in Eccrine Sweat Glands. J. Invest. Dermatol. 125, 428–444 (2005).

Species human

Application IHC (frozen)

Publication Herrmann, T. et al. Mice with targeted disruption of the fatty acid transport protein 4 (Fatp 4, Slc27a4) gene show features of lethal restrictive dermopathy. J. Cell Biol. 161, 1105–1115 (2003).

Species mouse

Application IHC (paraffin)

Publication Langbein, L. et al. Tight junction-related structures in the absence of a lumen: occludin, claudins and tight junction plaque proteins in densely packed cell formations of stratified epithelia and squamous cell carcinomas. Eur. J. Cell Biol. 82, 385–40

Species human

Application IHC (frozen)

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File IHC guinea pig antibody

Category Protocol

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Datasheet

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FAQs

PVDF membranes show better results than nitrocellulose (higher capacity, allows for more stringent washing conditions in case of background problems).
Use freshly prepared blocking solution (e.g. 5% nonfat dry milk, 0.05% Tween 20), block for at least 1 h at room temperature.
Use the antibody in a higher dilution, but prolong incubation time and exposure time.
Always use a fresh aliquot of the antibody.
Do not repeatedly freeze the antibody (eventually centrifuge shortly after thawing to remove cryo-precipitates).
Include an additional washing step.
You might also try more stringent wash conditions, e.g. add 0.5 M NaCl to the wash buffer.
Always use a fresh aliquot of secondary antibody.
In case you use ECL most the guinea pig antibody should be diluted further in order to get rid of the background.

Most of our purified mouse antibodies contain 0.5% BSA as stabilizer. If BSA was added to the antibody solution, it is stated in the datasheet.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.

Lyophilized antibodies can be stored at 2-8°C until expiration.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.

The expiration date of our antibodies is indicated on the product label.

Isolation of positive area from tissue slices under the microscope, followed by extraction procedure to enrich for the cytoskeleton.
Positive tissue: outer root sheath of hair follicle, sweat gland epithelium on the foot pads, filiform papillae of tongue.

Homogenization (e.g. with polytron) of tissue samples in buffer L (140 mM NaCl, 5 mM EDTA, 5 mM EGTA, 10 mM Tris-HCl pH7.6) supplemented with protease inhibitors; use 1 ml buffer for approx. 0.1 g tissue.
To reduce viscosity by high DNA contents, benzonase treatment can be included (30 min at 37°C).
Centrifugation for 10 min (max. speed, table top centrifuge).
Resuspend pellet in "Triton extraction buffer" (buffer L plus 1% Triton X-100) using e.g. a dounce homogenizer.
Centrifugation for 10 min (max. speed, table top centrifuge).
Resuspend pellet in "high salt extraction buffer" (buffer L plus 1.5 M KCl) using e.g. a dounce homogenizer.
Centrifugation for 10 min (max. speed, table top centrifuge).
Resuspend pellet in "Triton extraction buffer" (buffer L plus 1% Triton X-100) using e.g. a dounce homogenizer.
Centrifugation for 10 min (max. speed, table top centrifuge).
Resuspend pellet in Laemmli buffer and boil (5 min) for SDS-PAGE.

Methanol/ acetone fixation: Immerse slide in precooled (-20°C) methanol for 5 min, immerse in precooled (-20°C) acetone for 30-60 sec, let specimen air dry before antibody incubation.
Methanol/ acetone fixation plus detergent permeabilization: After methanol/ acetone fixation and air-drying dip slide either in a solution containing 0.1-0.2% Triton X-100 in PBS or in 0.1% saponin in PBS for 1-5 min at room temperature (enhances accessibility of many cytoskeletal antigens).

Air-drying of the section.
Block with the serum of the species in which the secondary antibody was raised for 30 min.
Incubation with 1st antibody 1 h at RT in moist chamber.
Wash 3x with PBS.
Incubation with appropriate fluorescent secondary antibody, 30-60 min at RT.
Wash 3x with PBS.
Immerse shortly into ethanol.
Let air dry.
Cover with mounting medium.

Most of our antibodies contain 0.09% sodium azide as preservative. If a preservative is added, it is mentioned in the datasheet.

The optimal antibody dilution for your specific protocol and application needs to be titrated in your lab with your equipment and sample. The optimal dilution may vary between protocols and samples. A good dilution for starting the titration is the dilution mentioned in the datasheet. If the sample needs a specific treatment (eg. Antigen retrieval for IHC on FFPE sections) this should also be mentioned on the antibody datasheet.

PROGEN antibodies are shipped at ambient temperature. The antibodies are stable at ambient temperature for the shipment period. Please store the antibodies as indicated in the datasheet upon arrival.

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anti-Keratin K14 guinea pig polyclonal, serum

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Protocol for cytokeratin enrichment for WB

Protocol for frozen sections with guinea pig antibodies

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