anti-Keratin K15 guinea pig polyclonal, serum

Cat. No: GP-CK15

Available, delivery time 1-7 days

$311.00*

Key Features
  • Guinea pig polyclonal
  • Suitable for IHC and WB
  • Reacts with human and mouse
Product description
Quantity 100 µl
Antibody Type Polyclonal
Host Guinea pig
Conjugate Unconjugated
Application IHC, WB
Purification Stabilized antiserum
Reactivity Human, Mouse
Storage Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use Research use only
Immunogen C-terminal tail region (including parts of the rod domain) of human recombinant keratin K15
Formulation Contains 0.09% sodium azide and 0.5% BSA
UniprotID P19012 (Human),Q61414 (Mouse)
Synonym Keratin, type I cytoskeletal 15, Cytokeratin-15, CK-15, Keratin-15, K15, KRT15, KRTB
Note Centrifuge prior to opening
Applications
Tested applications Tested dilutions
Immunohistochemistry (IHC) - frozen 1:100-1:200
Western Blot (WB) 1:2,000
Background

Mr 50 kDa (pI 4.9) intermediate filament polypeptide, keratin K15 (formerly also designated cytokeratin 15), detected by immunofluorescence microscopy in basal cells of stratified epithelia (including epidermis, tongue, esophagus, exocervix) and complex stratified epithelia (e.g. broncheal epithelium), in luminal epithelia of mammary, salivary and sweat gland. In the hair follicle, staining was restricted to the outermost cell layer of the outer root sheath; within the hair follicle several cell layers of the hair follicle bulb were positive with antiserum gp 15.1.

In human fetal tissues keratin K15 was localized in basal cells of epidermis, lung, trachea, tracheal glands and esophagus. In fetal hair anlagen, all cells were positive with antiserum gp 15.1, suggesting that keratin K15 is preferentially expressed in epithelial stem cells. Completely unreactive were cells of liver, colon, kidney, urinary bladder, pancreas and lung alveolus.

In tumors, gp15.1 is reactive with squamous skin and lung carcinomas and with a significant subgroup of invasive ductal breast carcinomas, especially tumors with higher grade malignancy.

Reactivity on cultured cell lines: HaCat, A-431; negative on PLC.

References/Publications (1)
Publication
Species
Application
Downloads
Q & A's

Please enter a valid email address.
Please enter a valid question.
Fields marked with * are mandatory.

There aren't any asked questions yet.
Customer Reviews

0 of 0 reviews

Leave a review!

Share your experiences with other customers.


anti-guinea pig IgG goat polyclonal, HRP conjugate
Cat. No: 90001
  • Goat polyclonal
  • Suitable for ELISA and WB
  • Reacts with guinea pig

$109.00*
anti-Keratin K15 guinea pig polyclonal, serum
Cat. No: GP-K15
  • Guinea pig polyclonal
  • Suitable for IHC
  • Reacts with human and mouse

$311.00*