Disease related
Highly published

anti-Keratin K18 mouse monoclonal, Ks18.04, lyophilized, purified

Liquid

Liquid

Lyophilized

Prediluted

Sample

FITC

Biotin

Cat. No: 61028

Available, delivery time 1-7 days

$304.00*

Key Features
  • Purified, lyophilized
  • Mouse monoclonal
  • Suitable for ICC/IF, IHC and WB
  • Reacts with bovine, dog, hamster, human, mouse, pig, rat, sheep, trout, zebrafish
  • Isotype: IgG1
Product description
Quantity 50 µg
Antibody Type Monoclonal
Host Mouse
Isotype IgG1
Conjugate Unconjugated
Application ICC/IF, IHC, WB
Purification Affinity chromatography
Reactivity Bovine, Dog, Hamster, Human, Mouse, Pig, Rat, Sheep, Trout, Zebrafish
Storage before reconstitution 2-8°C until indicated expiry date
Storage after reconstitution Up to 3 months at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use Research use only
Clone Ks18.04
Immunogen Human keratin K18 from HeLa cytoskeletal preparation
Concentration 50 µg/ml after reconstitution with 1 ml dist. water
Formulation Lyophilized; reconstitute in 1 ml dist. water (final solution contains 0.09% sodium azide, 0.5% BSA in PBS buffer, pH 7.4)
UniprotID A1XEA5 (Bovine),P05783 (Human),P05784 (Mouse),F1SGG1 (Pig),Q5BJY9 (Rat),W5Q5M3 (Sheep)
Synonym Keratin, type I cytoskeletal 18, Cell proliferation-inducing gene 46 protein, Cytokeratin-18, CK-18, Keratin-18, K18, KRT18, CYK18, PIG46
Applications
Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) Assay dependent
Immunohistochemistry (IHC) - frozen 1:20-1:200
Immunohistochemistry (IHC) - paraffin 1:20-1:200 (microwave treatment recommended)
Western Blot (WB) 1:100-1:500 (0.5 µg/ml-0.1 µg/ml)
Background

Ks18.04 represents an excellent marker to discriminate simple epithelia from those of different origin. Tumors specifically detected: all adenocarcinoma; mammary carcinoma, urinary bladder carcinoma, undifferentiated carcinoma, cervix carcinoma, hepatocellular carcinoma. Polypeptide reacting: Mr 45,000 polypeptide (human keratin K18; formerly also designated cytokeratin 18) of all simple type epithelia and basal cells of many squamous, nonepidermal epithelia.

Tested cultured cell lines: MCF-7.

References/Publications (25)
Publication
Species
Application
Downloads
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Category
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Category Protocol
Size 924.86 KB
Filetype pdf
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FAQs
The concentration of unpurified supernatant, ascites, unpurified guinea pig serum and unpurified rabbit serum is not determined.
The concentration of purified antibodies is mentioned on the datasheet.
For prediluted antibodies the concentration may vary from lot to lot. The concentration of these antibodies is not mentioned on the datasheet and can be requested at support@progen.com.

Most of our purified mouse antibodies contain 0.5% BSA as stabilizer. If BSA was added to the antibody solution, it is stated in the datasheet.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.

Please find the solvent for reconstitution and the amount of liquid for reconstitution on the antibody datasheet. The buffer formulation after reconstitution is also mentioned in the datasheet.
PROGEN offers different formats for mouse monoclonal antibodies.  Different antibodies may not be offered in each format, the following are all possible formats:
  1. Supernatant and supernatant concentrate: This format contains hybridoma cell culture supernatant. The antibody is not purified and the antibody concentration is not determined. The antibody concentration may vary from lot to lot. Therefore we recommend to titrate the optimal concentration for the application used for each new lot.
  2. Lyophilized, purified: This format contains purified antibody in lyophilized form. The reconstitution of this antibody is described in the datasheet. The buffer composition after reconstitution is also mentioned on the datasheet. 
  3. Liquid, purified: This format contains purified antibody in liquid format. The concentration is mentioned on the datasheet. 
  4. Prediluted, purified: This format contains purified antibody in liquid format. Most antibodies in this format are diluted to be ready-to-use for IHC with standard tissue. But some antibodies of this format need further dilution for IHC. This is mentioned on the datasheet.

Lyophilized antibodies can be stored at 2-8°C until expiration.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.

Most of our antibodies contain 0.09% sodium azide as preservative. If a preservative is added, it is mentioned in the datasheet.
The optimal antibody dilution for your specific protocol and application needs to be titrated in your lab with your equipment and sample. The optimal dilution may vary between protocols and samples. A good dilution for starting the titration is the dilution mentioned in the datasheet. If the sample needs a specific treatment (eg. Antigen retrieval for IHC on FFPE sections) this should also be mentioned on the antibody datasheet. 
PROGEN antibodies are shipped at ambient temperature. The antibodies are stable at ambient temperature for the shipment period. Please store the antibodies as indicated in the datasheet upon arrival.
Positive western blot control: anti-Keratin K18 antibody
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  • Validated and tested western blot control for: anti-Keratin K18 mouse monoclonal antibody, clone Ks18.04, Cat. No. 61028 and Cat. No. 65028

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anti-Keratin K18 guinea pig polyclonal, serum
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  • Suitable for ICC/IF, IHC and WB
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anti-Keratin K18 guinea pig polyclonal, serum
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  • Guinea pig polyclonal
  • Suitable for ICC/IF, IHC and WB
  • Reacts with bovine, human, mouse and rat

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