Epitope mapped
Frequently used
Diagnostic marker

anti-Keratin K19 mouse monoclonal, Ks19.1 (A53-B/A2), prediluted, purified

Liquid

Prediluted

Lyophilized

Liquid

Cat. No: 65010

Available, delivery time 1-7 days

$191.00*

Key Features
  • Purified, prediluted
  • Mouse monoclonal
  • Suitable for ICC/IF, IHC and WB
  • Reacts with human
  • Isotype: IgG2a
Product description
Quantity 5 ml
Antibody Type Monoclonal
Host Mouse
Isotype IgG2a
Conjugate Unconjugated
Application ICC/IF, IHC, WB
Purification Affinity chromatography
Reactivity Human
Storage Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use Research use only
Clone Ks19.1 (A53-B/A2)
Immunogen Keratin K19 of Mr 40 000; from cultured human MCF-7 cells
Formulation PBS pH 7.4 with 0.5% BSA and 0.09% sodium azide
UniprotID P08727 (Human)
Synonym Keratin, type I cytoskeletal 19, Cytokeratin-19, CK-19, Keratin-19, K19, KRT19
Applications
Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) Assay dependent
Immunohistochemistry (IHC) - frozen Ready-to-use
Immunohistochemistry (IHC) - paraffin Ready-to-use (microwave treatment recommended)
Western Blot (WB) Assay dependent
Background

Ks 19.1 represents an excellent marker to discriminate glandular epithelial carcinoma from those of different origin. No reaction with hepatocellular carcinoma. Polypeptide reacting: Mr 40,000 polypeptide (keratin K19; formerly also designated cytokeratin 19) of human glandular epithelia. The epitope has been localized on aa. 311-335 (QSQLSMKAALEDTLAETEARFGAQL) of the alpha-helical fragment.

Tumors specifically detected: all tested adenocarcinoma; cholangio carcinoma of liver; renal cell carcinoma; transitional cell carcinoma of the bladder; ovary carcinoma; squamous cell carcinoma of cervix, bronchus and lung (intermediate type); mesothelioma; carcinoid tumor of bronchus; breast carcinoma; thymoma.

Reactivity on cultured cell lines: MCF-7.

References/Publications (18)
Publication
Species
Application
Downloads
File
Category
Size
Filetype
Category Protocol
Size 924.86 KB
Filetype pdf
Category Protocol
Size 919.99 KB
Filetype pdf
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