Epitope mapped
Frequently used
Diagnostic marker

anti-Keratin K19 mouse monoclonal, Ks19.2 (Z105.6), lyophilized, purified

Liquid

Lyophilized

Liquid

Sample

Cat. No: 61029

Available, delivery time 1-7 days

$276.00*

Key Features
  • Purified, lyophilized
  • Mouse monoclonal
  • Suitable for ELISA, ICC/IF, IHC and WB
  • Reacts with bovine, human, rabbit, rat
  • Isotype: IgG2b
Product description
Quantity 50 µg
Antibody Type Monoclonal
Host Mouse
Isotype IgG2b
Conjugate Unconjugated
Application ELISA, ICC/IF, IHC, WB
Purification Affinity chromatography
Reactivity Bovine, Human, Rabbit, Rat
No reactivity Chicken, Mouse, Woodchuck, Xenopus
Storage before reconstitution 2-8°C until indicated expiry date
Storage after reconstitution Up to 3 months at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use Research use only
Clone Ks19.2 (Z105.6)(also published as BM 19.21, MAK 19.21)
Immunogen Keratin K19 of Mr 40 000; from cultured human MCF-7 cells
Concentration 50 µg/ml after reconstitution with 1 ml dist. water
Formulation Lyophilized; reconstitute in 1 ml dist. water (final solution contains 0.09% sodium azide, 0.5 % BSA in PBS buffer, pH 7.4)
UniprotID P08728 (Bovine), P08727 (Human), Q63279 (Rat)
Synonym Keratin, type I cytoskeletal 19, Cytokeratin-19, CK-19, Keratin-19, K19, KRT19
Applications
Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) Assay dependent
Immunohistochemistry (IHC) - frozen 1:10-1:50
Immunohistochemistry (IHC) - paraffin 1:10-1:50 (microwave treatment recommended)
ELISA Assay dependent
Western Blot (WB) 1:50-1:500
Background

Ks 19.2 represents an excellent marker to discriminate glandular epithelial carcinoma from those of different origin. No reaction with hepatocellular carcinoma! Polypeptide Reacting: Mr 40,000 polypeptide (keratin K19; formerly also designated cytokeratin 19) of human glandular epithelia. The epitope has been localized on aa. 352-368 (VRADSERQNQEYQRLMD) of the alpha-helical fragment.

Tumors specifically detected: all tested adenocarcinoma; cholangio carcinoma of liver; renal cell carcinoma; transitional cell carcinoma of the bladder; ovary carcinoma; squamous cell carcinoma of cervix, bronchus and lung (intermediate type); mesothelioma; carcinoid tumor of bronchus; breast carcinoma; thymoma.

Reactivity on cultured cell lines: MCF-7.

References/Publications (19)
Publication
Species
Application
Downloads
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Category Protocol
Size 924.86 KB
Filetype pdf
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