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anti-Keratin K2 mouse monoclonal, Ks2.342.7.4, supernatant

Human skin (courtesy of J.Heß, University Hospital Heidelberg)
Human skin (courtesy of J.Heß, University Hospital Heidelberg)
Rat tail (courtesy of J.Heß, University Hospital Heidelberg)
Rat tail (courtesy of J.Heß, University Hospital Heidelberg)
Cat. No: 65191

Prices excl. tax

Available, delivery time: 1-7 days

$179.00*

Datasheet
Key Features
  • Mouse monoclonal
  • Suitable for ICC/IF and IHC
  • Reacts with bovine and human
  • Isotype: IgG1
Product description
Quantity 1 ml
Antibody Type Monoclonal
Host Mouse
Isotype IgG1
Conjugate Unconjugated
Application ICC/IF, IHC, WB
Purification Hybridoma cell culture supernatant
Reactivity Bovine, Human
Storage Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use Research use only
Clone Ks2.342.7.4
Immunogen Synthetic peptide (N-terminal amino acids nos. 2-23) of human keratin K2 (MW 65,852)
Formulation Contains 0.09% sodium azide
Stability Bovine: calf hoof epidermis, snooze epithelium; negative with tongue epithelium; Mouse/rat: heterogenously on foot sole epidermis only, negative with epidermis of other body sites.
UniprotID G3MZ71 (Bovine), P35908 (Human)
Synonym Keratin, type II cytoskeletal 2 epidermal, Cytokeratin-2e, CK-2e, Epithelial keratin-2e, Keratin-2 epidermis, Keratin-2e, K2e, Type-II keratin Kb2, KRT2, KRT2A, KRT2E
Note Centrifuge prior to opening
Applications
Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) 1:200
Immunohistochemistry (IHC) - frozen 1:100-1:250 (pretreatment with Triton-X100 recommended)
Immunohistochemistry (IHC) - paraffin 1:250-1:500 (microwave treatment recommended)
Background

Ks 2.342.7.4 represents an excellent marker to study terminal epidermal differentiation. The mab is reactive with epidermal cells in uppermost suprabasal layers including scalp, foot and sole. It recognizes individual cells within epidermis of tongue and mamille (co-localization with keratin K10). It is also reactive on hyperkeratosis of diverse viral and genetic origin. The mab does not react with palate keratin K76.

The actual clone designation of this antibody is Ks2.342.7.4. The two last digits refer to the passage number of the cultured cells of clone Ks2.342. Passage 7.1 is used up.

Reactive polypeptide (specificity): basic human keratin K2 (MW 65,852; formerly also designated cytokeratin 2e).

Antigen recognized in species:

  • Bovine: calf hoof epidermis, snooze epithelium; negative with tongue epithelium
  • Mouse/rat: heterogenously on foot sole epidermis only, negative with epidermis of other body sites
References/Publications (1)
Publication
Species
Application
Publication Langbein, L. et al. New facets of keratin K77: Interspecies variations of expression and different intracellular location in embryonic and adult skin of humans and mice. Cell Tissue Res. 354, 793–812 (2013).
Species human
Application IHC (frozen)
Downloads
File
Category
Size
Filetype
File IHC mouse antibody
Category Protocol
Size 167.19 KB
Filetype pdf
Datasheet
MSDS
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FAQs
The concentration of unpurified supernatant, ascites, unpurified guinea pig serum and unpurified rabbit serum is not determined.
The concentration of purified antibodies is mentioned on the datasheet.
For prediluted antibodies the concentration may vary from lot to lot. The concentration of these antibodies is not mentioned on the datasheet and can be requested at support@progen.com.

Most of our purified mouse antibodies contain 0.5% BSA as stabilizer. If BSA was added to the antibody solution, it is stated in the datasheet.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.

The monoclonal antibody to cytokeratin 2e (cat. no. 65191) is very sensitive, however, it might be possible that the antigen is a very minor component in the sample. In such a case enrichment (like a cytoskeletal preparation of the starting tissue) is highly recommended, also (if possible) to get rid of most lipidic or contaminating connective tissue (e.g. when working with epidermal tissue). The positive detection in cultured cells (e.g. HaCaT keratinocytes) is only possible by microscopical methods, since only very few individual cells are expressing the antigen. Detection by Western blotting is not sensitive enough in that case.
PROGEN offers different formats for mouse monoclonal antibodies.  Different antibodies may not be offered in each format, the following are all possible formats:
  1. Supernatant and supernatant concentrate: This format contains hybridoma cell culture supernatant. The antibody is not purified and the antibody concentration is not determined. The antibody concentration may vary from lot to lot. Therefore we recommend to titrate the optimal concentration for the application used for each new lot.
  2. Lyophilized, purified: This format contains purified antibody in lyophilized form. The reconstitution of this antibody is described in the datasheet. The buffer composition after reconstitution is also mentioned on the datasheet. 
  3. Liquid, purified: This format contains purified antibody in liquid format. The concentration is mentioned on the datasheet. 
  4. Prediluted, purified: This format contains purified antibody in liquid format. Most antibodies in this format are diluted to be ready-to-use for IHC with standard tissue. But some antibodies of this format need further dilution for IHC. This is mentioned on the datasheet.

Lyophilized antibodies can be stored at 2-8°C until expiration.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.

The expiration date of our antibodies is indicated on the product label.
We offer bulk amounts starting from 1 mg for many of our mouse monoclonal or recombinant antibodies. Please contact us for further information or use the “bulk button” on the product page.
Most of our antibodies contain 0.09% sodium azide as preservative. If a preservative is added, it is mentioned in the datasheet.
The optimal antibody dilution for your specific protocol and application needs to be titrated in your lab with your equipment and sample. The optimal dilution may vary between protocols and samples. A good dilution for starting the titration is the dilution mentioned in the datasheet. If the sample needs a specific treatment (eg. Antigen retrieval for IHC on FFPE sections) this should also be mentioned on the antibody datasheet. 
PROGEN antibodies are shipped at ambient temperature. The antibodies are stable at ambient temperature for the shipment period. Please store the antibodies as indicated in the datasheet upon arrival.

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Keratin

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