Frequently used
Diagnostic marker

anti-Keratin K20 mouse monoclonal, IT-Ks20.8, liquid, purified, small

Liquid

Liquid

Lyophilized

Prediluted

Prediluted

Cat. No: 690026S

Available, delivery time 1-7 days

$170.00*

Key Features
  • Purified, liquid, small
  • Mouse monoclonal
  • Suitable for IHC and WB
  • Reacts with human and mouse
  • Isotpye: IgG2a
Product description
Quantity 200 µl (50 µg/ml)
Antibody Type Monoclonal
Host Mouse
Isotype IgG2a
Conjugate Unconjugated
Application IHC, WB
Purification Affinity chromatography
Reactivity Human, Mouse
Storage Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use Research use only
Clone IT-Ks20.8
Immunogen Electrophoretically purified keratin K20 from human intestinal mucosa
Concentration 50 µg/ml
Formulation PBS buffer, pH 7.4 with 0.09% sodium azide, 0.5% BSA
UniprotID P35900 (Human), Q9D312 (Mouse)
Synonym Keratin, type I cytoskeletal 20, Cytokeratin-20, CK-20, Keratin-20, K20, Protein IT, KRT20
Applications
Tested applications Tested dilutions
Immunohistochemistry (IHC) - frozen 1:10-1:100 (treatment with 0.02% Triton X-100 recommended)
Immunohistochemistry (IHC) - paraffin 1:10-1:100 (protease treatment and/or microwave treatment recommended)
Western Blot (WB) 1:1,000-1:5,000
Background

IT-Ks20.8 represents an excellent marker for certain types of carcinomas such as adenocarcinomas of the colon, transitional cell carcinomas of the bladder and Merkel cell tumors of the skin. Very sensitive detection of intestinal and gastric foveolar epithelium, urothelial umbrella cells, Merkel cells of epidermis as well as tumors originating therefrom (e.g. primary and metastatic colorectal carcinoma). Adenocarcinomas of breast, lung, endometrium and ovary (non-mucinous) as well as neuroendocrine tumors of the lung are essentially negative.
Polypeptide recognized: protein IT (keratin K20; Mr 46,000; formerly also designated cytokeratin 20).

Tested cultured cell lines: RT-4.

References/Publications (7)
Publication
Species
Application
Downloads
File
Category
Size
Filetype
Category Protocol
Size 924.86 KB
Filetype pdf
Category Protocol
Size 919.99 KB
Filetype pdf
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