anti-Keratin K3/K76 mouse monoclonal, AE5, purified

Cat. No: 61807

Available, delivery time 1-7 days

$439.00*

Key Features
  • Mouse monoclonal
  • Suitable for IHC and WB
  • Reacts with bovine, human and rabbit
  • Isotype: IgG1
Product description
Quantity 200 µg (1 mg/ml)
Antibody Type Monoclonal
Host Mouse
Isotype IgG1
Conjugate Unconjugated
Application IHC, WB
Purification Affinity chromatography
Reactivity Bovine, Human, Rabbit
Storage Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use Research use only
Clone AE5
Immunogen Human epidermal keratin
Concentration 1 mg/ml

Formulation PBS with 0.09% sodium azide
UniprotID A0A3Q1MYR8 (Bovine),P12035 (Human),Q29426 (Rabbit)
Synonym Keratin, type II cytoskeletal 3, 65 kDa cytokeratin, Cytokeratin-3, CK-3, Keratin-3, K3, Type-II keratin Kb3, KRT3
Note Centrifuge prior to opening
Applications
Tested applications Tested dilutions
Immunohistochemistry (IHC) - frozen 1:50
Western Blot (WB) 1:500
Background

AE 5 represents an excellent marker for corneal type differentiation. Positive for epithelial cells of cornea, snout and some oral mucosa. This antibody has been used for studying corneal epithelial stem cells.
Polypeptide reacting: Mr 64,000 polypeptide (keratin K3; formerly also designated cytokeratin 3) of human corneal epithelium and keratin K76 (formerly also designated cytokeratin K2p) of palate epithelium.
Tested cultured cell lines: rabbit corneal epithelial cells.

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FAQs
The concentration of unpurified supernatant, ascites, unpurified guinea pig serum and unpurified rabbit serum is not determined.
The concentration of purified antibodies is mentioned on the datasheet.
For prediluted antibodies the concentration may vary from lot to lot. The concentration of these antibodies is not mentioned on the datasheet and can be requested at support@progen.com.

Most of our purified mouse antibodies contain 0.5% BSA as stabilizer. If BSA was added to the antibody solution, it is stated in the datasheet.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.

Lyophilized antibodies can be stored at 2-8°C until expiration.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.

The expiration date of our antibodies is indicated on the product label.
Isolation of positive area from tissue slices under the microscope, followed by extraction procedure to enrich for the cytoskeleton.
Positive tissue: outer root sheath of hair follicle, sweat gland epithelium on the foot pads, filiform papillae of tongue.
  • Homogenization (e.g. with polytron) of tissue samples in buffer L (140 mM NaCl, 5 mM EDTA, 5 mM EGTA, 10 mM Tris-HCl pH7.6) supplemented with protease inhibitors; use 1 ml buffer for approx. 0.1 g tissue.
  • To reduce viscosity by high DNA contents, benzonase treatment can be included (30 min at 37°C).
  • Centrifugation for 10 min (max. speed, table top centrifuge).
  • Resuspend pellet in "Triton extraction buffer" (buffer L plus 1% Triton X-100) using e.g. a dounce homogenizer.
  • Centrifugation for 10 min (max. speed, table top centrifuge).
  • Resuspend pellet in "high salt extraction buffer" (buffer L plus 1.5 M KCl) using e.g. a dounce homogenizer.
  • Centrifugation for 10 min (max. speed, table top centrifuge).
  • Resuspend pellet in "Triton extraction buffer" (buffer L plus 1% Triton X-100) using e.g. a dounce homogenizer.
  • Centrifugation for 10 min (max. speed, table top centrifuge).
  • Resuspend pellet in Laemmli buffer and boil (5 min) for SDS-PAGE.

Most of our antibodies contain 0.09% sodium azide as preservative. If a preservative is added, it is mentioned in the datasheet.
The optimal antibody dilution for your specific protocol and application needs to be titrated in your lab with your equipment and sample. The optimal dilution may vary between protocols and samples. A good dilution for starting the titration is the dilution mentioned in the datasheet. If the sample needs a specific treatment (eg. Antigen retrieval for IHC on FFPE sections) this should also be mentioned on the antibody datasheet. 
PROGEN antibodies are shipped at ambient temperature. The antibodies are stable at ambient temperature for the shipment period. Please store the antibodies as indicated in the datasheet upon arrival.

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