Epitope mapped

anti-Keratin K5/K8 (Pan Epithelial) mouse monoclonal, C22, lyophilized, purified

Liquid

Liquid

Lyophilized

Biotin

FITC

Prediluted

Cat. No: 61031

Available, delivery time 1-7 days

$289.00*

Key Features
  • Purified, lyophilized
  • Mouse monoclonal
  • Suitable for ICC/IF, IHC and WB
  • Reacts with bovine, human, mouse and rat
  • Isotype: IgG1
Product description
Quantity 50 µg
Antibody Type Monoclonal
Host Mouse
Isotype IgG1
Conjugate Unconjugated
Application ICC/IF, IHC, WB
Purification Affinity chromatography
Reactivity Bovine, Human, Mouse, Rat
Storage before reconstitution 2-8°C until indicated expiry date
Storage after reconstitution Up to 3 months at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use Research use only
Clone Ks 5+8.22/C22
Immunogen Human keratin K8, purified from SDS PAGE gel
Concentration 50 µg/ml after reconstitution with 1 ml dist. water
Formulation Lyophilized; reconstitute in 1 ml dist. water (final solution contains 0.09% sodium azide, 0.5% BSA in PBS buffer, pH 7.4)
UniprotID Q5XQN5 (Bovine), Q7RTS7 (Human), Q922U2 (Mouse), P05786 (Bovine), P05787 (Human), P11679 (Mouse)
Synonym Keratin, type II cytoskeletal 74, Cytokeratin-74, CK-74, Keratin-5c, K5C, Keratin-74, K74, Type II inner root sheath-specific keratin-K6irs4, Type-II keratin Kb37, KRT74, K6IRS4, KB37, KRT5C, KRT6IRS4, Keratin, type II cytoskeletal 8, Cytokeratin-8, CK-8, Keratin-8, K8, Type-II keratin Kb8, KRT8, CYK8
Applications
Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) Assay dependent
Immunohistochemistry (IHC) - frozen 1:10-1:100 (0.5-5 µg/ml)
Immunohistochemistry (IHC) - paraffin 1:10-1:100 (0.5-5 µg/ml, protease treatment and/or microwave treatment recommended)
Western Blot (WB) 1:500 (0.1 µg/ml)
Background

C22 represents an excellent marker for distinguishing carcinomas from all non-epithelial tumors. The antibody specifically reacts with keratins K5 and K8 present in nearly all epithelia.

Polypeptide reacting: Mr 52,500, Mr 58,000 keratins (type II keratins K5 and K8; formerly also designated cytokeratins 5 and 8) of human epithelial cells. Epitope has been mapped to aa 353-367 on alpha helical rod domain of Keratin K8 (Waseem et al., 2004).

Reactivity on cultured cell lines: MCF-7, RT 112, HT-29, HaCaT, Detroit 562, RPMI 2650, SSC-12, bovine BMGE+H, BMGE-H, MDBK.

Waseem A, Karsten U, Leigh IM, Purkis P, Waseem NH, Lane BE: Conformational changes in the rod domain of human keratin 8 following heterotypic association with keratin 18 and its implication for filament stability. Biochemistry 43, 1283-1295 (2004).

References/Publications (3)
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FAQs
The concentration of unpurified supernatant, ascites, unpurified guinea pig serum and unpurified rabbit serum is not determined.
The concentration of purified antibodies is mentioned on the datasheet.
For prediluted antibodies the concentration may vary from lot to lot. The concentration of these antibodies is not mentioned on the datasheet and can be requested at support@progen.com.

Most of our purified mouse antibodies contain 0.5% BSA as stabilizer. If BSA was added to the antibody solution, it is stated in the datasheet.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.

Please find the solvent for reconstitution and the amount of liquid for reconstitution on the antibody datasheet. The buffer formulation after reconstitution is also mentioned in the datasheet.
PROGEN offers different formats for mouse monoclonal antibodies.  Different antibodies may not be offered in each format, the following are all possible formats:
  1. Supernatant and supernatant concentrate: This format contains hybridoma cell culture supernatant. The antibody is not purified and the antibody concentration is not determined. The antibody concentration may vary from lot to lot. Therefore we recommend to titrate the optimal concentration for the application used for each new lot.
  2. Lyophilized, purified: This format contains purified antibody in lyophilized form. The reconstitution of this antibody is described in the datasheet. The buffer composition after reconstitution is also mentioned on the datasheet. 
  3. Liquid, purified: This format contains purified antibody in liquid format. The concentration is mentioned on the datasheet. 
  4. Prediluted, purified: This format contains purified antibody in liquid format. Most antibodies in this format are diluted to be ready-to-use for IHC with standard tissue. But some antibodies of this format need further dilution for IHC. This is mentioned on the datasheet.

Lyophilized antibodies can be stored at 2-8°C until expiration.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.

The expiration date of our antibodies is indicated on the product label.
We offer bulk amounts starting from 1 mg for many of our mouse monoclonal or recombinant antibodies. Please contact us for further information or use the “bulk button” on the product page?
Isolation of positive area from tissue slices under the microscope, followed by extraction procedure to enrich for the cytoskeleton.
Positive tissue: outer root sheath of hair follicle, sweat gland epithelium on the foot pads, filiform papillae of tongue.
  • Homogenization (e.g. with polytron) of tissue samples in buffer L (140 mM NaCl, 5 mM EDTA, 5 mM EGTA, 10 mM Tris-HCl pH7.6) supplemented with protease inhibitors; use 1 ml buffer for approx. 0.1 g tissue.
  • To reduce viscosity by high DNA contents, benzonase treatment can be included (30 min at 37°C).
  • Centrifugation for 10 min (max. speed, table top centrifuge).
  • Resuspend pellet in "Triton extraction buffer" (buffer L plus 1% Triton X-100) using e.g. a dounce homogenizer.
  • Centrifugation for 10 min (max. speed, table top centrifuge).
  • Resuspend pellet in "high salt extraction buffer" (buffer L plus 1.5 M KCl) using e.g. a dounce homogenizer.
  • Centrifugation for 10 min (max. speed, table top centrifuge).
  • Resuspend pellet in "Triton extraction buffer" (buffer L plus 1% Triton X-100) using e.g. a dounce homogenizer.
  • Centrifugation for 10 min (max. speed, table top centrifuge).
  • Resuspend pellet in Laemmli buffer and boil (5 min) for SDS-PAGE.

Most of our antibodies contain 0.09% sodium azide as preservative. If a preservative is added, it is mentioned in the datasheet.
The optimal antibody dilution for your specific protocol and application needs to be titrated in your lab with your equipment and sample. The optimal dilution may vary between protocols and samples. A good dilution for starting the titration is the dilution mentioned in the datasheet. If the sample needs a specific treatment (eg. Antigen retrieval for IHC on FFPE sections) this should also be mentioned on the antibody datasheet. 
PROGEN antibodies are shipped at ambient temperature. The antibodies are stable at ambient temperature for the shipment period. Please store the antibodies as indicated in the datasheet upon arrival.
anti-Keratin K5/K8 (Pan Epithelial) mouse monoclonal, C22, prediluted, purified
Cat. No: 65031
Quantity: 5 ml
  • Purified, prediluted
  • Mouse monoclonal
  • Suitable for IHC and WB
  • Reacts with bovine, human, mouse and rat
  • Isotype: IgG1

Variants from $96.00*
$220.00*
anti-Keratin K19 mouse monoclonal, Ks19.1 (A53-B/A2), liquid, purified, sample
Cat. No: 690010S
Quantity: 200 µl (50 µg/ml)
  • Purified, liquid, small
  • Mouse monoclonal
  • Suitable for ELISA, ICC/IF, IHC and WB
  • Reacts with human
  • Isotype: IgG2a

$96.00*
anti-Keratin K20 mouse monoclonal, IT-Ks20.8, liquid, purified, sample
Cat. No: 690026S
Quantity: 200 µl (50 µg/ml)
  • Purified, liquid, small
  • Mouse monoclonal
  • Suitable for IHC and WB
  • Reacts with human and mouse
  • Isotpye: IgG2a

$96.00*
anti-Keratin K8 mouse monoclonal, Ks8.7, liquid, purified
Cat. No: 690038
Quantity: 1 ml (50 µg/ml)
  • Purified, liquid
  • Mouse monoclonal
  • Suitable for ICC/IF, IHC and WB
  • Reacts with hamster and human
  • Isotype: IgG1

Variants from $96.00*
$251.00*
anti-Keratin K7 mouse monoclonal, Ks7.18, liquid, purified, sample
Cat. No: 690025S
Quantity: 200 µl (50 µg/ml)
  • Purified, liquid, small
  • Mouse monoclonal
  • Suitable for ICC/IF, IHC and WB
  • Reacts with bovine, human, pig and sheep
  • Isotype: IgG1

$96.00*
anti-Keratin K18 mouse monoclonal, Ks18.04, FITC Conjugate
Cat. No: 61428
Quantity: 250 µl
  • FITC conjugate
  • Mouse monoclonal
  • Suitable for ICC/IF, IHC
  • Reacts with bovine, dog, hamster, human, mouse, pig, rat, sheep, trout, zebrafish
  • Isotype: IgG1

Variants from $96.00*
$342.00*
anti-Keratin K19 mouse monoclonal, Ks19.2 (Z105.6), liquid, purified
Cat. No: 690029
Quantity: 1 ml (50 µg/ml)
  • Purified, liquid
  • Mouse monoclonal
  • Suitable for ELISA, ICC/IF, IHC and WB
  • Reacts with bovine, human, rabbit, rat
  • Isotype: IgG2b

Variants from $96.00*
$251.00*
anti-Keratin K17 mouse monoclonal, Ks17.E3, liquid, purified
Cat. No: 690036
Quantity: 1 ml (50 µg/ml)
  • Purified, liquid
  • Mouse monoclonal
  • Suitable for IHC and WB
  • Reacts with human, rat
  • Isotype: IgG2b

Variants from $96.00*
$251.00*
anti-Keratin K2 mouse monoclonal, Ks2.398.3.1, supernatant
Cat. No: 65177
Quantity: 1 ml
  • Hybridoma cell culture supernatant
  • Mouse monoclonal
  • Suitable for IHC and WB
  • Reacts with human
  • Isotype: IgG1

$179.00*
anti-Keratin K2 mouse monoclonal, Ks2.342.7.4, supernatant
Cat. No: 65191
  • Mouse monoclonal
  • Suitable for ICC/IF and IHC
  • Reacts with bovine and human
  • Isotype: IgG1

$179.00*
anti-Keratin K19 mouse monoclonal, Ks19.10, supernatant
Cat. No: 65135
  • Mouse monoclonal
  • Suitable for ELISA, ICC/IF, IHC and WB
  • Reacts with bovine, human and rat
  • Isotype: IgG1

$191.00*
anti-Keratin K8 mouse monoclonal, Ks8-17.2, supernatant
Cat. No: 65130
  • Mouse monclonal
  • Suitable for ICC/IF, IHC and WB
  • Reacts with bovine, hamster, human and rat
  • Isotype: IgG1

$191.00*
anti-Keratin K18 mouse monoclonal, Ks18.5, supernatant
Cat. No: 65137
  • Mouse monoclonal
  • Suitable for ELISA, ICC/IF, IHC and WB
  • Reacts with bovine and human
  • Isotype: IgG1

$191.00*
anti-Keratin K7 + K17 mouse monoclonal, C46, supernatant
Cat. No: 18313
  • Mouse monoclonal
  • Suitable for ICC/IF and IHC on frozen and paraffin sections
  • Reacts with bovine, dog, human and sheep
  • Isotype: IgG1

$215.00*
anti-Keratin K7 mouse monoclonal, OVTL 12-30, supernatant
Cat. No: 18315
  • Mouse monoclonal
  • Suitable for ICC/IF and IHC on frozen and paraffin sections
  • Reacts with human
  • Isotype: IgG1

$215.00*
anti-Keratin Pan mouse monoclonal, Lu-5, lyophilized, purified
Cat. No: 610145
  • Purified, lyophilized
  • Mouse monoclonal
  • Suitable for IHC
  • Reacts with bovine, chicken, human, mouse, pleurodeles, rat, snake and xenopus
  • Isotype: IgG1

$216.00*
anti-Keratin K20 mouse monoclonal, IT-Ks20.10, lyophilized, purified
Cat. No: 61054
Quantity: 50 µg
  • Purified, lyophilized
  • Mouse monoclonal
  • Suitable for ELISA, IHC and WB
  • Reacts with human, mouse and pig
  • Isotype: IgG1

Variants from $225.00*
$371.00*
anti-Keratin Pan rabbit polyclonal, purified
Cat. No: 10550
  • Rabbit Polyclonal
  • Suitable for IHC on frozen and  paraffin sections
  • Reacts with human and mouse

$232.00*
anti-Keratin K9 mouse monoclonal, Ks9.70 + Ks9.216, supernatant
Cat. No: 651104
  • Mouse monoclonal
  • Suitable for ICC/IF, IHC and WB
  • Reacts with human
  • Isotypes: IgG1 and IgG3

$245.00*
anti-Keratin K6 mouse monoclonal, KA12, lyophilized, purified
Cat. No: 61090
Quantity: 50 µg
  • Purified, lyophilized
  • Mouse monoclonal
  • Suitable for IHC and WB
  • Reacts with human, mouse and rat
  • Isotype: IgG1

Variants from $96.00*
$251.00*
anti-Keratin K18 mouse monoclonal, Ks18.27, lyophilized, purified
Cat. No: 61034
  • Mouse monoclonal
  • Suitable for ELISA, IHC and WB
  • Reacts with bovine and human
  • Isotype: IgG1

$251.00*
anti-Keratin K13 mouse monoclonal, Ks13.1, lyophilized, purified
Cat. No: 61007
Quantity: 50 µg
  • Purified, lyophilized
  • Mouse monoclonal
  • Suitable for ICC/IF, IHC and WB
  • Reacts with bovine, human, rat
  • Isotype: IgG1

Variants from $161.00*
$251.00*
anti-Keratin K18 Multiepitope Cocktail mouse monoclonal, Ks18.04 + 18.27 + 18.5 + 9B1 + 18.174, supernatant
Cat. No: 651134
  • Mouse monoclonal
  • Suitable for ICC/IF, IHC and WB
  • Reacts with human and mammals
  • Isotype: IgG1

$258.00*
anti-Keratin Type I mouse monoclonal, AE1, purified
Cat. No: 61804
  • Mouse monoclonal
  • Suitable for ICC/IF,  IHC and WB
  • Reacts with bovine, chicken, dog, human, monkey, mouse, rabbit and rat
  • Isotype: IgG1

$284.00*