Disease related

anti-Keratin K7 mouse monoclonal, Ks7.18, prediluted, purified

Liquid

Liquid

Lyophilized

Prediluted

Cat. No: 65025

Available, delivery time 1-7 days

$161.00*

Key Features
  • Purified, prediluted
  • Mouse monoclonal
  • Suitable for ICC/IF, IHC and WB
  • Reacts with bovine, human, pig and sheep
  • Isotype: IgG1
Product description
Quantity 5 ml
Antibody Type Monoclonal
Host Mouse
Isotype IgG1
Conjugate Unconjugated
Application ICC/IF, IHC, WB
Purification Affinity chromatography
Reactivity Bovine, Human, Pig, Sheep
No reactivity Dog, Mouse, Rabbit, Rat
Storage Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use Research use only
Clone Ks7.18
Immunogen Cytoskeletal proteins from cultured HeLa cells
Formulation PBS pH 7.4 with 0.5% BSA and 0.09% sodium azide
UniprotID Q29S21 (Bovine), P08729 (Human), A0A287ASI0 (Pig), H0VIA2 (Guinea pig), A0A6P7DW90 (Sheep)
Synonym Keratin, type II cytoskeletal 7, Cytokeratin-7, CK-7, Keratin-7, K7, Sarcolectin, Type-II keratin Kb7, KRT7, SCL
Applications
Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) Assay dependent
Immunohistochemistry (IHC) - frozen Ready-to-use
Immunohistochemistry (IHC) - paraffin Ready-to-use (protease treatment and/ or microwave treatment recommended)
Western Blot (WB) Assay dependent
Background

Ks7.18 represents an excellent marker for the discrimination of specific subtypes of adenocarcinoma: e.g. adenocarcinoma of pancreas, bile duct carcinoma and transitional carcinoma of bladder are stained, whereas hepatocellular and prostate carcinomas are negative.
Detects specific subtypes of adenocarcinomas: adenocarcimona of pancreas, gallbladder, lung, cervix; cholangio carcinoma of liver; ductal and lobular carcinoma of breast; carcinomas of ovary; transitional cell carcinoma of bladder; mesothelioma; negative with most cases of hepatocellular carcinoma. In colorectal carcinoma early stages are reported to be negative but advanced stages of tumor development are positive for keratin K7 expression. Occasionally, staining of blood vessel walls, particularly of endothelial cells may be observed.
Reacts with Mr 54,000 polypeptide (keratin K7; formerly also designated cytokeratin 7) of human glandular epithelia.

Reactivity on cultured cell lines: HeLa, RT 112, T24, BT-20, CAMA-1, Detroit 562, (MCF-7 and HT-29 are negative).

References/Publications (1)
Publication
Species
Application
Downloads
File
Category
Size
Filetype
Category Protocol
Size 924.86 KB
Filetype pdf
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anti-Keratin K7 mouse monoclonal, OVTL 12-30, supernatant
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  • Mouse monoclonal
  • Suitable for ICC/IF and IHC on frozen and paraffin sections
  • Reacts with human
  • Isotype: IgG1

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anti-Keratin K7 guinea pig polyclonal, serum
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  • Suitable for ICC/IF, IHC and WB
  • Reacts with human

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