Diagnostic marker

anti-Keratin K7 mouse monoclonal, OVTL 12-30, supernatant

Cat. No: 18315

Available, delivery time 1-7 days

$215.00*

Key Features
  • Mouse monoclonal
  • Suitable for ICC/IF and IHC on frozen and paraffin sections
  • Reacts with human
  • Isotype: IgG1
Product description
Quantity 1 ml
Antibody Type Monoclonal
Host Mouse
Isotype IgG1
Conjugate Unconjugated
Application ICC/IF, IHC
Purification Hybridoma cell culture supernatant
Reactivity Human
Storage Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use Research use only
Clone OVTL 12-30
Immunogen Cytoskeletal preparation from T24 cells
Formulation Contains 0.09% sodium azide
UniprotID P08729 (Human)
Synonym Keratin, type II cytoskeletal 7, Cytokeratin-7, CK-7, Keratin-7, K7, Sarcolectin, Type-II keratin Kb7, KRT7, SCL
Note Centrifuge prior to opening
Applications
Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) Assay dependent
Immunohistochemistry (IHC) - frozen 1:5-1:10
Immunohistochemistry (IHC) - paraffin 1:5-1:10 (microwave treatment recommended)
Background

The monoclonal antibody OVTL 12-30 can be used to distinguish between ovarian carcinomas and colon carcinomas in routinely processed tissue sections. Immunoblotting : The antibody is reactive with the 54 kD cytokeratin protein (cytokeratin 7).

Positive control: Glandular epithelium breast

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FAQs
The concentration of unpurified supernatant, ascites, unpurified guinea pig serum and unpurified rabbit serum is not determined.
The concentration of purified antibodies is mentioned on the datasheet.
For prediluted antibodies the concentration may vary from lot to lot. The concentration of these antibodies is not mentioned on the datasheet and can be requested at support@progen.com.

Most of our purified mouse antibodies contain 0.5% BSA as stabilizer. If BSA was added to the antibody solution, it is stated in the datasheet.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.

Lyophilized antibodies can be stored at 2-8°C until expiration.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.

The expiration date of our antibodies is indicated on the product label.
Most of our antibodies contain 0.09% sodium azide as preservative. If a preservative is added, it is mentioned in the datasheet.
The optimal antibody dilution for your specific protocol and application needs to be titrated in your lab with your equipment and sample. The optimal dilution may vary between protocols and samples. A good dilution for starting the titration is the dilution mentioned in the datasheet. If the sample needs a specific treatment (eg. Antigen retrieval for IHC on FFPE sections) this should also be mentioned on the antibody datasheet. 
PROGEN antibodies are shipped at ambient temperature. The antibodies are stable at ambient temperature for the shipment period. Please store the antibodies as indicated in the datasheet upon arrival.
anti-Keratin K7 mouse monoclonal, Ks7.18, lyophilized, purified
Cat. No: 61025
Quantity: 50 µg
  • Purified, lyophilized
  • Mouse monoclonal
  • Suitable for ICC/IF, IHC and WB
  • Reacts with bovine, human, pig and sheep
  • Isotype: IgG1

Variants from $96.00*
$239.00*
anti-Keratin K7 guinea pig polyclonal, serum
Cat. No: GP-K7
  • Guinea pig polyclonal
  • Suitable for ICC/IF, IHC and WB
  • Reacts with human

$311.00*