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anti-Keratin K75 guinea pig polyclonal, serum

anti-Keratin K75 guinea pig polyclonal, serum
Keratin K75 (green) and Keratin K1 (red) staining on human hair root View more
anti-Keratin K75 guinea pig polyclonal, serum
Human scalp hair (courtesy of L. Langbein) View more
anti-Keratin K75 guinea pig polyclonal, serum
Human scalp hair (courtesy of L. Langbein) View more
anti-Keratin K75 guinea pig polyclonal, serum
anti-Keratin K75 guinea pig polyclonal, serum
anti-Keratin K75 guinea pig polyclonal, serum
Cat. No: GP-K6HF

Prices excl. tax

Available, delivery time 1-7 days

$325.00*

Datasheet
Key Features
  •     Stabilized antiserum
  •     Guinea pig polyclonal
  •     Suitable for IHC and WB
  •     Reacts with human and mouse
Product description
Quantity 100 µl
Antibody Type Polyclonal
Host Guinea pig
Conjugate Unconjugated
Application IHC, WB
Purification Stabilized antiserum
Reactivity Human, Mouse
Storage Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use Research use only
Immunogen Synthetic peptide of human type II keratin Human K75 (formerly also designated K6hf) (FTTSGGHSLGAGLGGSGFSATSNRGL-C)
Formulation Contains 0.09% sodium azide and 0.5% BSA
Note Centrifuge prior to opening
Applications
Tested applications Tested dilutions
Immunohistochemistry (IHC) - frozen 1:100-1:200
Western Blot (WB) 1:2,000
Background
The antiserum stains specifically the companion layer (innermost layer of the outer root sheath) of the hair follicle. Although there is a considerable homology of keratin K75 (K6hf) to keratins K5 and K6, no cross-reactivity was observed, neither in Western blotting nor in immunofluorescence microscopy.
References/Publications (6)
Publication
Species
Application
Publication Geueke, A. et al. The anti‐apoptotic Bcl‐2 protein regulates hair follicle stem cell function. EMBO Rep. 22, 1–14 (2021).
Species Mouse
Application IHC-Fr-IF
Publication Langbein, L., Yoshida, H., Praetzel-Wunder, S., Parry, D. A. & Schweizer, J. The Keratins of the Human Beard Hair Medulla: The Riddle in the Middle. J. Invest. Dermatol. 130, 55-73 (2010).
Species human
Application IHC (frozen)
Publication Jennemann, R. et al. Integrity and Barrier Function of the Epidermis Critically Depend on Glucosylceramide Synthesis. J. Biol. Chem. 282, 3083-3094 (2006).
Species mouse
Application IHC (paraffin)
Publication Peitsch, W. K. et al. Drebrin, an Actin-Binding, Cell-Type Characteristic Protein: Induction and Localization in Epithelial Skin Tumors and Cultured Keratinocytes. J. Invest. Dermatol. 125, 761-774 (2005).
Species human
Application IHC (frozen)
Publication Langbein, L., Spring, H., Rogers, M. A., Praetzel, S. & Schweizer, J. Hair keratins and hair follicle-specific epithelial keratins. Methods Cell Biol 78, 413-451 (2004).
Species human
Application IHC (frozen),IEM
Publication Langbein, L. et al. A novel epithelial keratin, hK6irs1, is expressed differentially in all layers of the inner root sheath, including specialized huxley cells (Fl-gelzellen) of the human hair follicle. J. Invest. Dermatol. 118, 789-799 (2002).
Species human
Application IHC (frozen),IEM
Downloads
Datasheet
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FAQs
Most of our purified mouse antibodies contain 0.5% BSA as stabilizer. If BSA was added to the antibody solution, it is stated in the datasheet.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.

Lyophilized antibodies can be stored at 2-8°C until expiration.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.

The expiration date of our antibodies is indicated on the product label.
Most of our antibodies contain 0.09% sodium azide as preservative. If a preservative is added, it is mentioned in the datasheet.
The optimal antibody dilution for your specific protocol and application needs to be titrated in your lab with your equipment and sample. The optimal dilution may vary between protocols and samples. A good dilution for starting the titration is the dilution mentioned in the datasheet. If the sample needs a specific treatment (eg. Antigen retrieval for IHC on FFPE sections) this should also be mentioned on the antibody datasheet. 
PROGEN antibodies are shipped at ambient temperature. The antibodies are stable at ambient temperature for the shipment period. Please store the antibodies as indicated in the datasheet upon arrival.

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