Epitope mapped
Diagnostic marker

anti-Keratin K8/K18 mouse monoclonal, NCL-5D3, supernatant

Cat. No: 10502

Available, delivery time 1-7 days

$345.00*

Key Features
  • Mouse monoclonal
  • Suitable for ICC/IF, IHC on frozen and paraffin sections and WB
  • Reacts with human
  • Isotype: IgG1
Product description
Quantity 1 ml
Antibody Type Monoclonal
Host Mouse
Isotype IgG1
Conjugate Unconjugated
Application ICC/IF, IHC, WB
Purification Hybridoma cell culture supernatant
Reactivity Human
Storage Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use Research use only
Clone NCL-5D3
Immunogen Isolated from human breast cancer carcinoma cell line MCF-7
Formulation Contains 0.1% sodium azide
UniprotID P05787 (Human)
Synonym Keratin, type II cytoskeletal 8, Cytokeratin-8, CK-8, Keratin-8, K8, Type-II keratin Kb8, KRT8, CYK8
Note Centrifuge prior to opening
Applications
Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) 1:5-1:10
Immunohistochemistry (IHC) - frozen 1:5-1:10
Immunohistochemistry (IHC) - paraffin 1:5-1:10 (protease treatment and/or microwave treatment recommended)
Western Blot (WB) Assay dependent
Background

The antibody recognizes all simple epithelia including glandular epithelia. It does not react with stratified squamous epithelium. Useful for the differentiation between carcinomas and lymphomas. In immunoblotting (Western) NCL 5D3 is directed against the the 52.2 kDa keratin K8 (originally reported also to 45 kDa keratin K18; formerly also designated cytokeratins according to the Moll-catalogue). Epitope has been mapped to aa 353-367 on alpha helical rod domain of keratin K8 (Waseem et al., 2004).

Positive control: glandular epithelium.

Waseem A, Karsten U, Leigh IM, Purkis P, Waseem NH, Lane BE: Conformational changes in the rod domain of human keratin 8 following heterotypic association with keratin 18 and its implication for filament stability. Biochemistry 43, 1283-1295 (2004).

References/Publications (1)
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FAQs
The concentration of unpurified supernatant, ascites, unpurified guinea pig serum and unpurified rabbit serum is not determined.
The concentration of purified antibodies is mentioned on the datasheet.
For prediluted antibodies the concentration may vary from lot to lot. The concentration of these antibodies is not mentioned on the datasheet and can be requested at support@progen.com.

Most of our purified mouse antibodies contain 0.5% BSA as stabilizer. If BSA was added to the antibody solution, it is stated in the datasheet.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.

Lyophilized antibodies can be stored at 2-8°C until expiration.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.

The expiration date of our antibodies is indicated on the product label.
Most of our antibodies contain 0.09% sodium azide as preservative. If a preservative is added, it is mentioned in the datasheet.
The optimal antibody dilution for your specific protocol and application needs to be titrated in your lab with your equipment and sample. The optimal dilution may vary between protocols and samples. A good dilution for starting the titration is the dilution mentioned in the datasheet. If the sample needs a specific treatment (eg. Antigen retrieval for IHC on FFPE sections) this should also be mentioned on the antibody datasheet. 
PROGEN antibodies are shipped at ambient temperature. The antibodies are stable at ambient temperature for the shipment period. Please store the antibodies as indicated in the datasheet upon arrival.
Keratin K8, human recombinant, 250 µg
Cat. No: 62013
Quantity: 250 µg
  • Recombinant human keratin K8 (formerly also designated cytokeratin 8)
  • Protein standard

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Keratin K18, human recombinant, 250 µg
Cat. No: 62017
Quantity: 250 µg
  • Recombinant human keratin K18 (formerly also designated cytokeratin 18)
  • Protein standard

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anti-Keratin K8/K18 guinea pig polyclonal, serum
Cat. No: GP11
  • Stabilized antiserum
  • Guinea pig polyclonal
  • Suitable for ELISA, IHC and WB
  • Reacts with bovine, chicken, human, mouse, rat and hamster

$375.00*