Epitope mapped
Diagnostic marker

anti-Keratin K8/K18 mouse monoclonal, NCL-5D3, supernatant

Cat. No: 10502

Available, delivery time 1-7 days


Key Features
  • Mouse monoclonal
  • Suitable for ICC/IF, IHC on frozen and paraffin sections and WB
  • Reacts with human
  • Isotype: IgG1
Product description
Quantity 1 ml
Antibody Type Monoclonal
Host Mouse
Isotype IgG1
Conjugate Unconjugated
Application ICC/IF, IHC, WB
Purification Hybridoma cell culture supernatant
Reactivity Human
Storage Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use Research use only
Clone NCL-5D3
Immunogen Isolated from human breast cancer carcinoma cell line MCF-7
Formulation Contains 0.1% sodium azide
UniprotID P05787 (Human)
Synonym Keratin, type II cytoskeletal 8, Cytokeratin-8, CK-8, Keratin-8, K8, Type-II keratin Kb8, KRT8, CYK8
Note Centrifuge prior to opening
Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) 1:5-1:10
Immunohistochemistry (IHC) - frozen 1:5-1:10
Immunohistochemistry (IHC) - paraffin 1:5-1:10 (protease treatment and/or microwave treatment recommended)
Western Blot (WB) Assay dependent

The antibody recognizes all simple epithelia including glandular epithelia. It does not react with stratified squamous epithelium. Useful for the differentiation between carcinomas and lymphomas. In immunoblotting (Western) NCL 5D3 is directed against the the 52.2 kDa keratin K8 (originally reported also to 45 kDa keratin K18; formerly also designated cytokeratins according to the Moll-catalogue). Epitope has been mapped to aa 353-367 on alpha helical rod domain of keratin K8 (Waseem et al., 2004).

Positive control: glandular epithelium.

Waseem A, Karsten U, Leigh IM, Purkis P, Waseem NH, Lane BE: Conformational changes in the rod domain of human keratin 8 following heterotypic association with keratin 18 and its implication for filament stability. Biochemistry 43, 1283-1295 (2004).

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