Diagnostic marker

anti-Keratin K9 mouse monoclonal, Ks9.70 + Ks9.216, supernatant

Cat. No: 651104

Available, delivery time 1-7 days

$245.00*

Key Features
  • Mouse monoclonal
  • Suitable for ICC/IF, IHC and WB
  • Reacts with human
  • Isotypes: IgG1 and IgG3
Product description
Quantity 1 ml
Antibody Type Monoclonal
Host Mouse
Isotype IgG1 and IgG3
Conjugate Unconjugated
Application ICC/IF, IHC, WB
Purification Hybridoma cell culture supernatant
Reactivity Human
No reactivity Mouse
Storage Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use Research use only
Clone Ks9.70 + Ks9.216
Immunogen Synthetic peptides [(1) amino acids pos. 450-477 and (2) N-terminal amino acids nos. 4-28] of human keratin K9 (MW 62,129; formerly also designated cytokeratin 9)
Formulation Contains 0.09% sodium azide
UniprotID P35527 (Human)
Synonym Keratin, type I cytoskeletal 9, Cytokeratin-9, CK-9, Keratin-9, K9, KRT9
Note Centrifuge prior to opening
Applications
Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) 1:10
Immunohistochemistry (IHC) - frozen 1:20
Immunohistochemistry (IHC) - paraffin 1:20 (protease treatment and/or microwave treatment recommended)
Western Blot (WB) 1:25-1:300
Background

The antibody reagent represents an excellent marker to study palmoplantar epidermal distribution and differentiation. Specifically reactive in the middle/upper suprabasal layers (stratum spinosum/ granulosum) of the epidermis of palm and sole. K9 can be detected in primary cultures of palmoplantar keratinocytes when they shift to differentiation-promoting conditions and grow stratified (upper cells). K9 has not been found in normal, i.e. non-pathogenic, non-ridged epidermis, beside some minor cells surrounding the acrosyringeal ducts. No labelling has been found in epithelial cells of other stratified epithelia such as oesophagus or complex epithelia (e.g. urothelium) or in ductal or simple epithelia. Negative tissues include: muscle, liver and duodenum.

The antibody cocktail is an excellent new tool to characterize primary cultures of keratinocytes/skin transplants for application in burn treatment.

References/Publications (1)
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anti-Keratin K9 guinea pig polyclonal, serum
Cat. No: GP-CK9
  • Guinea pig polyclonal
  • Suitable for IHC and WB
  • Reacts with bovine, human, mouse and rat

$311.00*