anti-Keratin Pan guinea pig polyclonal + anti-guinea pig IgG HRP set
The anti-Keratin Pan antibody covers all types of epithelia and epithelium-derived neoplasms.
|Quantity||100 µl anti-Keratin Pan + 100 µg anti-guinea pig IgG HRP|
|Host||Goat, Guinea pig|
|Reactivity||See individual antibody datasheet|
|Storage||Lyophilized at 2-8°C; reconstituted at -20°C (avoid freeze/thaw cycles)|
|Intended use||Research use only|
|Immunogen||See individual antibody datasheet for information about specific immunogens|
|Formulation||Keratin Pan antibody: contains 0.09% sodium azide and 0.5% BSA; anti-guinea pig IgG HRP antibody: lyophilized, reconstitute in 500 µl dist. water (final solution contains 10 mg/ml BSA in PBS buffer, pH 7.4)|
|Note||Centrifuge prior to opening|
|Tested applications||Tested dilutions|
|Immunohistochemistry (IHC) - frozen||anti-Keratin Pan: 1:100-1:200|
|Immunohistochemistry (IHC) - paraffin||anti-Keratin Pan: 1:50-1:200 (microwave treatment recommended)|
|Western Blot (WB)||anti-Keratin Pan: 1:1,000-1:2,000; anti-guinea pig HRP: 1:2,500-1:100,000|
anti-Keratin Pan antibody:
Reactive Polypeptides: Acidic (K1, K4, K8) and basic (K14) keratins (40 - 68 kDa polypeptides).
Tumors specifically detected: All types of epithelia and epithelium-derived neoplasms.
anti-guinea pig IgG goat polyclonal, HRP conjugate:
Whole Guinea Pig IgG secondary antibodies are isolated from antisera by immunoaffinity chromatography. The antibody is conjugated to Horseradish peroxidase (HRP) and is commonly used for WB, IHC and ELISA.
Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule guinea pig IgG. It also reacts with the light chains of other guinea pig immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody may cross-react with immunoglobulins from other species.
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