Disease related
Conformational epitope
Frequently used

anti-Keratin Pan mouse monoclonal, Lu-5, lyophilized, purified

Cat. No: 610145

Available, delivery time 1-7 days

$216.00*

Key Features
  • Purified, lyophilized
  • Mouse monoclonal
  • Suitable for IHC
  • Reacts with bovine, chicken, human, mouse, pleurodeles, rat, snake and xenopus
  • Isotype: IgG1
Product description
Quantity 50 µg
Antibody Type Monoclonal
Host Mouse
Isotype IgG1
Conjugate Unconjugated
Application ICC/IF, IHC
Purification Affinity chromatography
Reactivity Bovine, Chicken, Human, Mouse, Pleurodeles, Rat, Snake, Xenopus
Storage before reconstitution 2-8°C
Storage after reconstitution Up to 3 months at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use Research use only
Clone Lu-5
Immunogen Cells of lung cancer cell line A549 and A2182
Concentration 50 µg/ml after reconstitution with 1 ml dist. water
Formulation Lyophilized; reconstitute in 1 ml dist. water (final solution contains 0.09% sodium azide, 0.5% BSA in PBS buffer, pH 7.4)
Applications
Tested applications Tested dilutions
Immunohistochemistry (IHC) - frozen Assay dependent
Immunohistochemistry (IHC) - paraffin 1:10-1:20 (protease treatment and/or microwave treatment recommended)
Western Blot (WB) Not applicable
Background

Lu-5 represents an excellent marker for all types of epithelia. Ideal marker for immunolocalization studies on frozen and paraffin embedded tissue. Useful for distinguishing carcinomas from non-epithelial tumors.
Tumors specifically detected: all epithelium-derived neoplasms.

Polypeptide reacting: Most keratins present in human epithelia (including keratins - formerly also designated cytokeratins -K1, K5, K6, K14, K18, K19) and most acidic (K31 - KK38) and basic (K81 - K86) hair keratins from human hair follicle. The antibody specifically detects a conformational epitope common to most keratins, but not to other intermediate filament polypeptides.

Reactivity on cultured cell lines: MCF-7, PLC, A-431, HeLa; PtK2, A6 (XLKE).

References/Publications (9)
Publication
Species
Application
Downloads
File
Category
Size
Filetype
Category Protocol
Size 924.86 KB
Filetype pdf
Q & A's

Please enter a valid email address.
Please enter a valid question.
Fields marked with * are mandatory.

There aren't any asked questions yet.
Customer Reviews

0 of 0 reviews

Leave a review!

Share your experiences with other customers.


anti-Keratin Pan rabbit polyclonal, purified
Cat. No: 10550
  • Rabbit Polyclonal
  • Suitable for IHC on frozen and  paraffin sections
  • Reacts with human and mouse

$232.00*
anti-Keratin Pan guinea pig polyclonal, serum
Cat. No: GP14
Quantity: 100 µl
  • Stabilized antiserum
  • Guinea pig polyclonal
  • Suitable for ICC/IF, IHC and WB
  • Reacts with human and mouse

Variants from $65.00*
$269.00*