anti-Keratin Pan mouse monoclonal, Lu-5, lyophilized, purified
- Purified, lyophilized
- Mouse monoclonal
- Suitable for IHC
- Reacts with bovine, chicken, human, mouse, pleurodeles, rat, snake and xenopus
- Isotype: IgG1
|Reactivity||Bovine, Chicken, Human, Mouse, Pleurodeles, Rat, Snake, Xenopus|
|Storage before reconstitution||2-8°C|
|Storage after reconstitution||Up to 3 months at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles|
|Intended use||Research use only|
|Immunogen||Cells of lung cancer cell line A549 and A2182|
|Concentration||50 µg/ml after reconstitution with 1 ml dist. water
|Formulation||Lyophilized; reconstitute in 1 ml dist. water (final solution contains 0.09% sodium azide, 0.5% BSA in PBS buffer, pH 7.4)|
|Tested applications||Tested dilutions|
|Immunohistochemistry (IHC) - frozen||Assay dependent|
|Immunohistochemistry (IHC) - paraffin||1:10-1:20 (protease treatment and/or microwave treatment recommended)|
|Western Blot (WB)||Not applicable|
Lu-5 represents an excellent marker for all types of epithelia. Ideal marker for immunolocalization studies on frozen and paraffin embedded tissue. Useful for distinguishing carcinomas from non-epithelial tumors.
Tumors specifically detected: all epithelium-derived neoplasms.
Polypeptide reacting: Most keratins present in human epithelia (including keratins - formerly also designated cytokeratins -K1, K5, K6, K14, K18, K19) and most acidic (K31 - KK38) and basic (K81 - K86) hair keratins from human hair follicle. The antibody specifically detects a conformational epitope common to most keratins, but not to other intermediate filament polypeptides.
Reactivity on cultured cell lines: MCF-7, PLC, A-431, HeLa; PtK2, A6 (XLKE).
Q & A's
0 of 0 reviews
- Stabilized antiserum
- Guinea pig polyclonal
- Suitable for ICC/IF, IHC and WB
- Reacts with human and mouse