Disease related
Frequently used

anti-Keratin Type I mouse monoclonal, AE1, purified

Cat. No: 61804

Available, delivery time 1-7 days

$271.00*

Key Features
  • Mouse monoclonal
  • Suitable for ICC/IF,  IHC and WB
  • Reacts with bovine, chicken, dog, human, monkey, mouse, rabbit and rat
  • Isotype: IgG1
Product description
Quantity 200 µg (1 mg/ml)
Antibody Type Monoclonal
Host Mouse
Isotype IgG1
Conjugate Unconjugated
Application ICC/IF, IHC, WB
Purification Affinity chromatography
Reactivity Bovine, Chicken, Dog, Human, Monkey, Mouse, Rabbit, Rat
Storage Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use Research use only
Clone AE1
Immunogen Human epidermal keratin (formerly also designated cytokeratin)
Concentration 1 mg/ml
Formulation PBS pH 7.4 with 0.09% sodium azide
Note Centrifuge prior to opening
Applications
Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) Assay dependent
Immunohistochemistry (IHC) - frozen 1:200
Immunohistochemistry (IHC) - paraffin 1:200 (protease treatment and/or microwave treatment recommended)
Western Blot (WB) Assay dependent
Background

AE 1 represents an excellent marker for distinguishing carcinoma from non-epithelial tumors. Tumors specifically detected: all epithelium-derived tumors.
Polypeptide reacting: Mr 40,000; Mr 48,000 and Mr 50,000 polypeptides of human epithelial proteins (keratins nos. K19, K16, K14) and most other acidic (type I) keratins.
Reactivity on cultured cell lines: MCF-7, A-431, RT 112.

References/Publications (15)
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anti-Keratin Type II mouse monoclonal, Ks pan1-8, lyophilized, purified
Cat. No: 61006
  • Mouse monoclonal
  • Suitable for ICC/IF, IHC and WB
  • Reacts with amphibia, bovine, human, mouse and rat
  • Isotype: IgG2a

$251.00*
anti-Keratin Type II mouse monoclonal, AE3, purified
Cat. No: 61806
  • Mouse monoclonal
  • Suitable for ICC/IF, IHC and WB
  • Reacts with bovine, chicken, human, monkey, mouse, rabbit and rat
  • Isotype: IgG1

$271.00*