anti-LDL Receptor rabbit polyclonal, purified

Serum

Biotin

Cat. No: 61099

Available, delivery time 1-7 days

$305.00*

Key Features
  • Purified serum
  • Rabbit polyclonal
  • Suitable for FACS, IHC, Receptor Binding Assays and WB
  • Reacts with bovine, human, mouse and rat
Product description
Quantity 250 µl (100 µg/ml)
Antibody Type Polyclonal
Host Rabbit
Isotype IgG
Conjugate Unconjugated
Application FACS, IHC, Receptor binding assay, WB
Purification Affinity chromatography
Reactivity Bovine, Human, Mouse, Rat
Storage Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use Research use only
Immunogen Specific synthetic peptide (sequence not conserved in VLDL receptor and LRP) of the human LDL receptor extracellular domain (epitope between residues nos. 184-195, the linker region between repeats 4 and 5)
Concentration 100 µg/ml
Formulation PBS with 0.5% BSA
Note Centrifuge prior to opening
Applications
Tested applications Tested dilutions
Immunohistochemistry (IHC) - frozen 1:20-1:50
Immunohistochemistry (IHC) - paraffin 1:20-1:50 (microwave treatment recommended)
Flow Cytometry (FACS) 1:10
Receptor Binding Assays Assay dependent
Western Blot (WB) 1:100-1:200
Background

The LDL receptor (160 kDa mature or glycosylated receptor; 120 kDa precursor or unglycosylated receptor) plays a key role in cellular cholesterol homeostasis.
The antibody to LDLR reacts specifically with human LDLR and is suitable for use in flow cytometry and immunoblotting. The antibody does not inhibit binding of LDL.
In immunoblotting, the antibody recognizes the 160 kDa band of LDLR and a 120 kDa band of the LDLR precursor from fibroblasts, hepatocytes, and monocytic cells cultured in the presence of lipoprotein-deficient serum.

References/Publications (5)
Downloads
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Category Protocol
Size 925.08 KB
Filetype pdf
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FAQs
The concentration of unpurified supernatant, ascites, unpurified guinea pig serum and unpurified rabbit serum is not determined.
The concentration of purified antibodies is mentioned on the datasheet.
For prediluted antibodies the concentration may vary from lot to lot. The concentration of these antibodies is not mentioned on the datasheet and can be requested at support@progen.com.

We do not recommend milk for blocking, since the antigen was also found in milk therefore .
Most of our purified mouse antibodies contain 0.5% BSA as stabilizer. If BSA was added to the antibody solution, it is stated in the datasheet.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.

Lyophilized antibodies can be stored at 2-8°C until expiration.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.

The expiration date of our antibodies is indicated on the product label.
Most of our antibodies contain 0.09% sodium azide as preservative. If a preservative is added, it is mentioned in the datasheet.
The optimal antibody dilution for your specific protocol and application needs to be titrated in your lab with your equipment and sample. The optimal dilution may vary between protocols and samples. A good dilution for starting the titration is the dilution mentioned in the datasheet. If the sample needs a specific treatment (eg. Antigen retrieval for IHC on FFPE sections) this should also be mentioned on the antibody datasheet. 
PROGEN antibodies are shipped at ambient temperature. The antibodies are stable at ambient temperature for the shipment period. Please store the antibodies as indicated in the datasheet upon arrival.
anti-rabbit IgG goat polyclonal, HRP conjugate
Cat. No: 90003
  • HRP conjugate
  • Goat polyclonal
  • Suitable for ELISA and WB
  • Reacts with rabbit

$109.00*
anti-LDL, human mouse monoclonal, M-34, lyophilized, purified
Cat. No: 61080
  • Purified, lyophilized
  • Mouse monoclonal
  • Reacts with human
  • Isotype: IgG2a

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anti-LDL Receptor mouse monoclonal, IgG-C7, lyophilized, purified
Cat. No: 61087
  • Mouse monoclonal
  • Suitable for FACS, IHC and ICC/IF
  • Reacts with bovine and human
  • Isotype: IgG2b kappa

$257.00*