Disease related

anti-MRP14/S100A9/calgranulin B mouse monoclonal, 47-8D3, purified

Cat. No: 691519

Available, delivery time 1-7 days


Key Features
  • Mouse monoclonal
  • Suitable for FACS, ICC/IF, IHC and WB
  • Reacts with cat, dog, goat, guinea pig, horse, human, monkey, pig, rabbit and rat
  • Isotype: IgG1 kappa
Product description
Quantity 1 ml (100 µg/ml)
Antibody Type Monoclonal
Host Mouse
Isotype IgG1 kappa
Conjugate Unconjugated
Application FACS, ICC/IF, IHC, WB
Purification Affinity chromatography
Reactivity Cat, Dog, Goat, Guinea pig, Horse, Human, Monkey, Pig, Rabbit, Rat
Storage 2-8°C
Intended use Research use only
Clone 47-8D3
Immunogen Human peripheral blood monocyte components derived by affinity chromatography with Sepharose 4B coupled to rabbit monocyte antibodies
Concentration 100 µg/ml
Formulation PBS with 0.02% sodium azide
UniprotID A0A452EXE8 (Goat), H0UWS6 (Guinea pig), F6T046 (Horse), P06702 (Human), F6Q697 (Pig), P50117 (Rabbit), P50117 (Rabbit), P50116 (Rat)
Synonym Protein S100-A9, Calgranulin-B, Calprotectin L1H subunit, Leukocyte L1 complex heavy chain, Migration inhibitory factor-related protein 14, MRP-14, p14, S100 calcium-binding protein A9, S100A9, CAGB, CFAG, MRP14
Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) 1:50-1:100 (1-2 µg/ml)
Immunohistochemistry (IHC) - frozen 1:50-1:100 (1-2 µg/ml)
Immunohistochemistry (IHC) - paraffin 1:50-1:100 (1-2 µg/ml; microwave treatment in 10 mM citrate buffer pH 6.0 or digestion with trypsin at 1 mg/ml PBS recommended)
Flow Cytometry (FACS) 1-2 µg/million cells in 0.1 ml, fix cells in 4% PFA for 10 min at 4°C, permeabilize with 0.2% saponin or digitonin for 15 min at 4°C
Western Blot (WB) 1:50-1:100 (1-2 µg/ml)

47-8D3 reacts with macrophages and detects the well-known leukocyte L1, a cystic fibrosis antigen. Detecting a single protein band of 14 kDa in Western blots of lysates of human monocytes and granulocytes, the antigen was identified as the calcium-binding protein MRP14, which is a member of the S100 family involved a.o. in regulating the cell cycle. MRP14 is also implicated in the abnormal differentiation of myeloid cells in the stroma of cancer. It is further found on squamous mucosal epithelia. When associated with MRP8 it forms the heterodimer calprotectin.

Positive control: monocytes, macrophages, granulocytes.

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