anti-Mucin mouse monoclonal, 45 MI, supernatant
- Mouse monoclonal
- Suitable for IHC on frozen and paraffin sections
- Reacts with human
- Isotype: IgG1
|Purification||Hybridoma cell culture supernatant|
|Storage||Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles|
|Intended use||Research use only|
|Immunogen||Mucin isolated from ovarian mucinous cysts and colonic mucosa|
|Formulation||Contains 0.09% sodium azide|
|Note||Centrifuge prior to opening|
|Tested applications||Tested dilutions|
|Immunohistochemistry (IHC) - frozen||1:10-1:40|
|Immunohistochemistry (IHC) - paraffin||1:10-1:40 (protease treatment and or microwave treatment recommended)|
The antibody is reactive with the peptide core of gastric mucin (MUC5AC, earlier also described as mucin M1). Mucin is an important tumor-associated marker for adenocarcinomas. The antibody shows specificity to gastric mucosa, but is also reactive with stomach and colon carcinomas.
Most breast carcinomas are negative.
The antigen is localized in the cytoplasm.
Positive control: Gastric mucosa of stomach.
Q & A's
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The concentration of purified antibodies is mentioned on the datasheet.
For prediluted antibodies the concentration may vary from lot to lot. The concentration of these antibodies is not mentioned on the datasheet and can be requested at firstname.lastname@example.org.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.